临床传统医学和药理学（英文）2025，Vol.6Issue(4)：67-74,8.DOI:10.1016/j.ctmp.2025.200248
银杏内酯B对干燥综合征小鼠模型干眼症的干预作用
Ginkgolide B alleviates dry eye in a Sjögren syndrome mouse model
摘要
Abstract
Background:Sjögren syndrome is an autoimmune disease characterized by inflammation of the exocrine glands,leading to dry eye disease.Current treatments have limited efficacy,indicating the need for novel therapeutic strategies. Objective:We aimed to investigate the therapeutic potential of ginkgolide B(GB)using a mouse model of Sjögren syndrome and explored the underlying molecular mechanisms. Methods:Female MRL/lpr mice were treated with 0.05％ GB,0.05％ cyclosporine A(CsA),or saline for 3 weeks.Treatment efficacy was evaluated by assessing tear secretion,ocular surface inflammation,and histological anal-ysis of the cornea and lacrimal gland.Mechanistically,mRNA expression levels of immune-regulated genes,in-cluding Il-4,Il-8,Tlr4,Nos2,Mmp9,Muc5ac,Tnf,Ifng,Il-1β,and Il-6 in the lacrimal glands were also determined. Results:Our results demonstrated that GB treatment significantly alleviated dry eye disease symptoms by increas-ing tear secretion and reducing ocular surface inflammation.Histological analyses revealed that GB protected both the corneal epithelium and lacrimal gland from pathological changes.RT-PCR results showed a significant down-regulation of nine inflammation-related molecules(Il-4,Il-8,Tlr4,Nos2,Muc5ac,Tnf,Ifng,Il-1β and Il-6),and upregulation of Mmp9 following GB treatment.Furthermore,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis identified IL-17 signalling pathway as the most enriched pathway. Conclusion:These findings suggest that GB effectively alleviates dry eye symptoms and may serve as a promising alternative to current treatments,possibly through modulation of IL-17 signalling pathway.关键词
银杏内酯B/干眼症/MRL/lpr小鼠/炎症Key words
Ginkgolide B/Dry eye/MRL/lpr mice/Inflammation引用本文复制引用
赵宸,吴廷标,陈玉川,杨振昊,文青,邹佳凤,李铭源,崔国祯..银杏内酯B对干燥综合征小鼠模型干眼症的干预作用[J].临床传统医学和药理学（英文）,2025,6(4):67-74,8.基金项目
This research was funded by the National Natural Science Founda-tion of China(No.82160684),China （No.82160684）
the Guizhou Province Science and Technology Program(No.ZK[2022]General 587),China （No.ZK[2022]General 587）
Innovation Team Project by the Department of Education of Guangdong Province(No.2024KCXTD005),China. （No.2024KCXTD005）
