临床与实验病理学杂志2026，Vol.42Issue(1)：38-45,55,9.DOI:10.13315/j.cnki.cjcep.2026.01.006
NANOG表达对食管鳞状细胞癌进展的促进作用
NANOG expression promotes progression in esophageal squamous cell carcinoma
摘要
Abstract
Objective To investigate the expression characteristics of NANOG in esophageal squamous cell car-cinoma(ESCC)tissues and its influence on the proliferation and migration of ESCC cells.Methods Immunohisto-chemistry was used to detect the expression of NANOG and Survivin in 107 cases of ESCC tissues and adjacent esopha-geal mucosal tissues and their correlations with clinicopathological features as well as patient prognosis were analyzed.A NANOG-overexpressing ESCC cell model(KYSE30)was constructed using lentiviral transfection.Cell prolifera-tion,migration,cell cycle distribution and apoptosis in KYSE30 cells were assessed using CCK-8,colony formation,scratch assay,Transwell chamber assay and flow cytometry.Western blot was performed to detect the effects of NANOG overexpression on the expression of E-cadherin,vimentin,Survivin,VEGF and Cyclin D1.Results NANOG cytoplasmic positivity was observed in 48.6%of ESCC cases,which was significantly negatively correlated with progression-free survival(P=0.001),but showed no significant association with overall survival(P=0.077).NANOG nuclear positivity was detected in 75.7%of ESCC cases,which was positively correlated with lymph node staging(P=0.003),but was not associated with overall survival or progression-free survival(P=0.961,P=0.972).Functional assays demonstrated that NANOG overexpression significantly promoted KYSE30 cell prolifera-tion and migration,increased the S-phase cell population,and suppressed apoptosis.Concurrently,it downregulated E-cadherin while upregulating the expression of vimentin,Survivin,VEGF,and Cyclin D1.Conclusion The cyto-plasmic positive expression of NANOG may serve as a biomarker for tumor progression in ESCC and is expected to be-come a potential therapeutic target for ESCC in the future.关键词
食管鳞状细胞癌/NANOG/Survivin/预后/增殖/迁移Key words
esophageal squamous cell carcinoma/NANOG/Survivin/prognosis/proliferation/migration分类
医药卫生引用本文复制引用
王慧,苏丽萍,李梦研,滕晓东..NANOG表达对食管鳞状细胞癌进展的促进作用[J].临床与实验病理学杂志,2026,42(1):38-45,55,9.基金项目
省部共建中亚高发病成因与防治国家重点实验室开发课题基金(SKL-HIDCA-2019-36)、省部共建中亚高发病成因与防治国家重点实验室开发课题基金(SKL-HIDCA-2021-27) State Key Laboratory of Pathogenesis,Prevention and Treatment of High Incidence Diseases in Cen-tral Asia Fund(SKL-HIDCA-2019-36) （SKL-HIDCA-2019-36）
State Key Laboratory of Pathogenesis,Prevention and Treatment of High Inci-dence Diseases in Central Asia Fund(SKL-HIDCA-2021-27) （SKL-HIDCA-2021-27）
