临床与实验病理学杂志2026，Vol.42Issue(1)：46-55,10.DOI:10.13315/j.cnki.cjcep.2026.01.007
c-Jun通过调控RNF144A-AS1转录影响食管鳞状细胞癌恶性生物学行为
c-Jun regulated the malignant biological behaviors of esophageal squamous cell carcinoma through transcriptional activation of RNF144A-AS1
摘要
Abstract
Objective This study aimed to investigate the role of c-Jun in regulating the transcription of RNF144A-AS1 and its impact on the proliferation,migration and invasion of esophageal squamous cell carcinoma(ESCC)cells.Methods Human esophageal epithelial cell(HEEpiC)and ESCC cell lines(Eca109,Kyse170,Kyse150 and TE1)were cultured under standard conditions.Knockdown sequences and overexpression plasmids were transfected into ESCC cells using transfection reagents.The expression of c-Jun mRNA and RNF144A-AS1 in ESCC tissues,different cell lines,and transfected Eca109 cells was detected by qRT-PCR.Western blotting was performed to evaluate the knockdown efficiency of c-Jun.Cell proliferation,migration,and invasion were examined using MTS,wound healing,and Transwell assays.Bioinformatic analysis was applied to predict the binding sites of c-Jun within the RNF144A-AS1 promoter region,which were further validated by ChIP and dual-luciferase reporter assays.Results qRT-PCR showed that RNF144A-AS1 and c-Jun expression levels in ESCC tissues[1.10(0.44,2.39);1.02(0.35,4.39)]were higher than in adjacent normal tissues[0.40(0.15,1.76);0.51(0.35,1.61),all P<0.05].Similarly,their expression levels in ESCC cell lines Eca109(15.54±1.74;15.14±1.52),Kyse170(10.92±0.70;9.62±0.86),Kyse150(8.91±0.18;7.68±0.46),and TE1(4.75±0.54;6.11±0.67)were markedly el-evated compared with HEEpiC cells(1.15±0.13;1.02±0.05,all P<0.01).GEPIA dataset analysis revealed a positive correlation between RNF144A-AS1 and c-Jun expression in esophageal cancer tissues(r=0.24,P=0.000 89),which was also observed in ESCC tissues(r=0.456,P<0.01).c-Jun protein expression was significantly correlated with pTNM stage(P<0.05).Knockdown of RNF144A-AS1 suppressed proliferation,migration,and invasion of Eca109 cells(P<0.05).siRNA-mediated c-Jun knockdown reduced RNF144A-AS1 expression,whereas c-Jun over-expression increased it(P<0.01).c-Jun directly bound to the RNF144A-AS1 promoter and transcriptionally acti-vated its expression(P<0.05).Overexpression of RNF144A-AS1 partially reversed the inhibitory effects of c-Jun knockdown on the malignant behaviors of Eca109 cells(P<0.05).Conclusion c-Jun promotes the malignant bio-logical behavior of ESCC cells by transcriptionally activating RNF144A-AS1.关键词
食管肿瘤/鳞状细胞癌/长链非编码RNA/转录调控/c-Jun/RNF144A-AS1Key words
esophageal neoplasms/squamous cell carcinoma/long non-coding RNA/transcriptional regulation/c-Jun/RNF144A-AS1分类
医药卫生引用本文复制引用
冯博,曹家瑞,许彦超,陈伟霞,马纯政..c-Jun通过调控RNF144A-AS1转录影响食管鳞状细胞癌恶性生物学行为[J].临床与实验病理学杂志,2026,42(1):46-55,10.基金项目
河南省重点研发与推广专项(科技攻关)项目(232102310126)、河南省中医院(河南中医药大学第二附属医院)博士科研基金(2021BSJJ04) Henan Provincial Key R&D and Promotion Special(Scientific and Technological Research)Project(232102310126) （科技攻关）
Henan Province Hospital of Traditional Chinese Medicine(the Second Affiliated Hospital of Henan University of Chinese Medicine)Doctoral Research Fund(2021BSJJ04) （the Second Affiliated Hospital of Henan University of Chinese Medicine）
