生物质化学工程2026，Vol.60Issue(1)：37-45,9.DOI:10.3969/j.issn.1673-5854.2026.01.005
桑叶总生物碱提取及其与牛血清蛋白结合能力研究
Extraction of Total Alkaloids From Mulberry Leaves and Its Binding With Bovine Serum Albumin
摘要
Abstract
The total alkaloids were extracted from mulberry leaves by ultrasonic low-temperature extraction process.Based on single factor experiments,the extraction process was optimized by response surface method.The main structures of total alkaloids from mulberry leaves were identified and analyzed by liquid chromatography/mass spectrometry,and the binding ability of total alkaloids from mulberry leaves to bovine serum albumin(BSA)was studied through a series of spectra.The results showed that the optimal extraction process of total alkaloids from mulberry leaves was as follows:mulberry leaves of 3.0 g,solid-liquid ratio of 0.05 mol/L hydrochloric acid solution to mulberry leaves10∶1(mL∶g),ethanol volume fraction of 33%,ultrasonic power of 250 W,extraction temperature of 40℃,extraction time of 30 min,and two times of extraction.Under these conditions,the extraction amount of total alkaloids from mulberry leaves was 2.596±0.13 mg/g.According to the analysis by liquid chromatography/mass spectrometry(LC/MS),there were four main structures of total alkaloids from mulberry leaves,2-O-α-D-galactopyranosyl-deoxynojirimycin,1-deoxynojirimycin(1-DNJ),1,4-dideoxy-1,4-imino-D-arabinitol and fagomine.It could be seen from the fluorescence spectrum that the total alkaloids from mulberry leaves could bind to BSA with a binding constant of(9.79±0.23)×103 L/(mol·s).Circular dichroism analysis confirmed that total alkaloids from mulberry leaves could alter the secondary structure of BSA.关键词
桑叶/总生物碱/牛血清蛋白/液相色谱/质谱联用/圆二色光谱Key words
mulberry leaves/total alkaloids/bovine serum albmin/HPLC-UV/MS chromatography/circular dichroic spectrum分类
化学化工引用本文复制引用
汤丽华,管勤昊,闫波雯,徐曼,张亮亮,胡新宇..桑叶总生物碱提取及其与牛血清蛋白结合能力研究[J].生物质化学工程,2026,60(1):37-45,9.基金项目
国家重点研发计划资助项目(2022YFD1300903) （2022YFD1300903）
