寄生虫与医学昆虫学报2025，Vol.32Issue(4)：198-209,12.DOI:10.3969/j.issn.1005-0507.2025.04.002
宁夏拉乌尔立克次体的分离鉴定及基因组特征分析
ISOLATION,IDENTIFICATION,AND GENOMIC CHARACTERIZATION OF RICKETTSIA RAOULTII FROM NINGXIA TICKS
摘要
Abstract
Objective Spotted fever group rickettsiae(SFGR)are globally prevalent obligate intracellular bacteria that have the potential to cause severe and fatal human diseases.A study to investigate the species and genetic characteristics of SFGR was conducted in 2023 in the Ningxia region of northwestern China.Methods Tick samples were collected from Ningxia,identified,and used to isolate SFGR.The isolated strain was identified as Rickettsia raoultii through morphological observation,multi-locus sequence typing(MLST),and phylogenetic analysis.Then,it was subjected to whole-genome sequencing using next-generation technology,which revealed it to be a TIGMIC strain.Results The TIGMIC strain was demonstrated to exhibit a capacity for growth in mammalian cell lines.The complete genome sequence of the TIGMIC strain was assembled into a circular chromosome,which exhibited a length of 1 312 176 bp and comprised 1 488 genes.Functional annotation revealed that the main key genes of the TIGMIC strain were primarily associated with biosynthesis,nutrient metabolism,and cell membrane biogenesis.Apart from the rickA gene,most typical rickettsial virulence genes of the TIGMIC strain were relatively conserved.Conclusions These findings will help identify SFGR,provide etiological evidence for spotted fever in Ningxia,and emphasize the need for enhanced surveillance to prevent the spread of SFGR.关键词
拉乌尔立克次体/分离/全基因组测序/比较基因组分析Key words
Rickettsia raoultii/Isolation/Genome sequencing/Comparative genomic analyses分类
医药卫生引用本文复制引用
朱雯杰,曹务春,余慧君,田娣,韩骁禺,王宁,沈世敬,林哲韬,王天虹,刘志宏..宁夏拉乌尔立克次体的分离鉴定及基因组特征分析[J].寄生虫与医学昆虫学报,2025,32(4):198-209,12.基金项目
国家重点研发计划(2021YFC2302001 （）
2023YFC2305901) （）
