甜味小鬼伞生物学特性、驯化栽培及抗氧化活性
Biological characteristics,domestic cultivation and antioxidant activities of Coprinellus saccharinus
摘要
Abstract
A Coprinellus saccharinus strain was isolated from mycelia on deciduous leaf humus in Nanshan Park(Qinglong County,Hebei,China).Four factors of biological characteristics were investigated.Fruiting bodies of this species were successfully cultivated.The scavenging capacities of both mycelium and fruiting body ethanol extracts against ABTS+·and·OH were also evaluated.The results indicated that the optimum carbon source for C.saccharinus strain were sucrose and soluble starch;the optimum nitrogen source was KNO3;the suitable pH range was 7-9,and the highest mycelial growth rate was observed at 25℃.Domestication experiment showed that bottleful mycelial colonization time required 20-25 d at(20±1)℃;primordium differentiation demanded temperature difference of 2-4℃,relative air humidity at 70％-80％,photoperoid of 12 h/d 800-1000 lx under scattered light,and a duration of 10-15 d.Mature fruiting bodies can be harvested after continued cultivation for 2-3 d.The antioxidant activities of C.saccharinus ethanol extracts from mycelia and cultivated fruiting bodies increased gradually as additive amount raising.Scavenging rates against both ABTS+·and·OH peaked at an additive volume of 200 μL.Scavenging rates of ethanol extract of cultivated fruiting bodies reached 97.93％and 67.22％,respectively,significantly higher than those of mycelia(64.73％and 24.74％).Both mycelium and cultivated fruiting body ethanol extracts exhibited stronger scavenging capacity against ABTS+·than·OH.Scavenging rate differentials were 39.99％and 30.71％,respectively.These findings provided a practical basis for further investigations into cultivation techniques,physiological activities,pharmacological effects,and other utilizations of C.saccharinus.关键词
药用真菌/生长条件/人工栽培Key words
medicinal fungus/growth condition/artificial cultivation引用本文复制引用
赵怡,康霞,常光雷,王立安,彭慧芬,田景花,李守勉,李国杰..甜味小鬼伞生物学特性、驯化栽培及抗氧化活性[J].菌物学报,2026,45(1):142-153,12.基金项目
河北省食用菌产业创新团队珍稀食用菌岗位(HBCT2023090202) （HBCT2023090202）
河北省自然科学基金(C2025204257) （C2025204257）
河北农业大学引进人才科研专项(YJ201849) （YJ201849）
国家食用菌产业技术体系小宗种类食用菌栽培岗位(CARS-20-4) （CARS-20-4）
河北省县域生物多样性调查评估项目(2011600012) （2011600012）
河北省科技支撑计划(2053731D) （2053731D）
河北省科技计划项目重点研发计划(21326315D) （21326315D）
河北省现代农业产业技术体系食用菌创新团队项目(HBCT2018050205) This work was supported by the Innovation Team of Edible Fungi of Hebei Modern Agricultural Industrial Technology System,Valuable and Rare Edible Fungus Position(HBCT2023090202),the Hebei Natural Science Foundation(C2025204257),the Talent Introduction Scientific Research Special Project of Hebei Agricultural University(YJ201849),the Earmarked Fund for China Agriculture Research System(CARS)-Edible Fungi,Non-staple Edible Fungus Species Cultivation Position(CARS-20-4),the Intra-County Biodiversity Investigation and Evaluation of Hebei Province(2011600012),the Hebei Province Science and Technology Support Plan(2053731D),the Key Research and Development Planning Project in Science and Technology of Hebei Province(21326315D),and the Innovation Team of Edible Fungi of Hebei Modern Agricultural Industrial Technology System(HBCT2018050205). （HBCT2018050205）
