生态与农村环境学报2026,Vol.42Issue(1):91-101,11.DOI:10.19741/j.issn.1673-4831.2024.0885
行间生草对葡萄园土壤养分、酶活性及微生物群落的影响
Effects of Inter-row Grass on Soil Nutrient,Enzyme Activity and Microbial Community in Vineyard
摘要
Abstract
This study investigated the effects of inter-row grass cropping on soil nutrients and microbial community struc-ture in wine grape vineyards in the eastern foothills of the Helan Mountains,aiming to identify suitable cover crop species for the region.Using Vitis vinifera L.cv."Chardonnay"as the test material,four cover crop species—Vicia sativa,Ono-brychis viciifolia,Echinochloa frumentacea,and Lolium perenne—were planted between vine rows,with traditional clean tillage as the control.The surface soil properties,enzyme activities,and microbial community structure were analyzed.The results showed that compared with clean tillage,the inter-row grass cropping reduced the soil pH value of the vine-yard,and the reduction range of Vicia sativa and Echinochloa frumentacea was the largest,with 12.83%and 13.40%,re-spectively.The contents of soil organic matter,total nitrogen,total phosphorus and available phosphorus were significantly increased in the inter-row grass cropping,and the best effects of Vicia sativa treatment were9.15g·kg-1,1.64g·kg-1,19.30 g·kg-1 and 72.61 mg·kg-1,respectively.The soil alkali hydrolyzable nitrogen(AN)content of Onobrychis vici-ifolia treatment was the highest,which was 35.53 mg·kg-1.Additionally,cover cropping enhanced the activities of soil enzymes,including catalase,urease,alkaline phosphatase,cellulase,sucrase,and amylase.Compared with clean till-age,the inter-row grass cropping increased the number of fungal and bacterial operational taxonomic units(OTUs).The Shannon,Simpson,and Chao1 indices of bacterial communities were elevated,while fungal community diversity showed no significant differences.The results of bacterial community composition showed that Proteobacteria(26.22%-32.98%),Bacteroidota(11.78%-16.83%)and Actinobacteriota(9.97%-17.29%)were the dominant phyla at the phylum level.Ascomycota(57.18%-85.11%)was the dominant phyla among fungi.Redundancy analysis indicated that amylase activity significantly influenced bacterial community structure,while AN content was the key factor affecting fungal communities.Principal component analysis(PCA)showed that the comprehensive scores of clean tillage,Vicia sativa,Onobrychis viciifolia,Echinochloa frumentacea and Lolium perenne were-2.268,1.954,1.455,-0.207 and-0.934,respectively,indicating that the best treatment effect is Vicia sativa.In conclusion,in the vineyards at the eastern foot of the Helan Mountains,inter-row grass cropping has certain improvement effects on soil physicochemical properties and en-zyme activities,increased the number of microorganisms in the soil,and changed the community structure and diversity of bacteria and fungi to varying degrees,among which the planting of Vicia sativa and Onobrychis viciifolia have a better effects on soil.关键词
行间生草/贺兰山东麓/土壤肥力/土壤酶活性/土壤微生物Key words
inter-row grass cropping/eastern foothills of the Helan Mountains/soil fertility/soil enzyme activity/soil microorganisms分类
农业科技引用本文复制引用
操敏,李明..行间生草对葡萄园土壤养分、酶活性及微生物群落的影响[J].生态与农村环境学报,2026,42(1):91-101,11.基金项目
贺兰山东麓酿酒葡萄精准种植与葡萄酒品质研究(SKL-IOW-2023TC2301) (SKL-IOW-2023TC2301)