农业生物技术学报2026，Vol.34Issue(2)：431-443,13.DOI:10.3969/j.issn.1674-7968.2026.02.016
表达IFN-γ的重组乳酸乳球菌的构建及其临床效果评价
Construction of Recombinant Lactococcus lactis Expressing IFN-γ and Evaluation of Its Clinical Efficacy
摘要
Abstract
Interferon-γ(IFN-γ)exhibits broad-spectrum antiviral activity and plays an important role in animal husbandry.This study aimed to construct a recombinant lactic acid bacterium expressing IFN-γ and evaluate its safety and therapeutic effect in clinical diarrheal diseases.Through molecular cloning technology,the goat IFN-γ gene was successfully inserted into the target plasmid pNZ8149,and the recombinant plasmid pNZ8149-IFN-γ was constructed.Furthermore,the recombinant Lactococcus lactis pNZ8149-IFN-γ/NZ3900(rL.lactis-IFN-γ)was successfully constructed.Methods such as restriction enzyme digestion identification,bacterial liquid PCR,and DNA sequencing were used to verify the correctness of the recombinant plasmid,and it was identified by Western blot that the recombinant strain could successfully express IFN-γ.The recombinant probiotic was evaluated for safety in animals such as cat(Felis silvestris catus)and goat(Capra hircus),and the results showed that the recombinant strain had no obvious toxicity to the experimental animals.The effect of the recombinant probiotic preparation was evaluated through naturally diseased animals(calves(Bos taurus)with persistent diarrhea,forest musk deer(Moschus berezovskii)with diarrhea,and dogs(Canis lupus familiaris)with gastroenteritis).The results showed that the recombinant probiotic preparation had a significant effect on the treatment of diarrhea in different species.The results of this study indicated that the recombinant lactic acid bacterium expressing IFN-γ could not only be safely applied in clinical practice,but also had a good effect in the treatment of animal diarrheal diseases.This study provides a new treatment strategy for animal diarrheal diseases and other related diseases caused by viral infections.关键词
乳酸乳球菌/干扰素γ(IFN-γ)/腹泻性疾病Key words
Lactococcus lactis/Interferon-γ(IFN-γ)/Diarrheal diseases分类
农业科技引用本文复制引用
王瑜琪,华进联,穆海龙,李娜,孙培文,王威,庞坤,王煜煜,吕长荣,孙文阳,梁小军,林永康..表达IFN-γ的重组乳酸乳球菌的构建及其临床效果评价[J].农业生物技术学报,2026,34(2):431-443,13.基金项目
宁夏回族自治区重点研发计划项目(2024BBF01014) （2024BBF01014）
宁夏回族自治区重点研发计划项目(2025BBF02007) （2025BBF02007）
内蒙古自治区揭榜挂帅项目(2022JBGUS0025) （2022JBGUS0025）
陕西省农业核心技术攻关(2024NYGG005-6) （2024NYGG005-6）
国家现代农业(肉牛牦牛)产业技术体系专项(CARS-37) （肉牛牦牛）
