农业与技术2026，Vol.46Issue(1)：11-18,8.DOI:10.19754/j.nyyjs.20260130003
耐寒月季开放式组培快繁体系的建立
Establishment of an Open Tissue Culture and Rapid Propagation System for Cold-Tolerant Roses
摘要
Abstract
By discussing the effects of bacteriostatic concentration and hormone combination on in vitro rapid propagation of cold-resistant rose,an open tissue culture rapid propagation system of cold-resistant rose"2021-MH"was established to provide technical support for industrialized seedling production of cold-resistant rose.A new cold-tolerant rose strain"2021-MH"was used as the material.At each stage of tissue culture and rapid propagation(primary culture,subculture and rooting culture),different concentration combinations(ZP antibiotics,Timentin and PPMTM)and plant growth hormones were added,and the effects of bacteriostatic agents and hormone combinations on open culture of cold-tolerant rose were comprehensively analyzed.The results indicate:The best combination of bacteriostatic agent and hormone concentration in the primary culture stage(bacteriostatic agent:60mg•L-1 ZP,100mL•L-1 Timentin,1.0mL•L-1 PPMTM;Medium:MS+1.5mg•L-1 6-BA+0.1mg•L-1 2,4-D+0.3mg•L-1 IBA),the germination rate was 96.15%,the contamination rate was 13.33%,the browning rate was 3.85%;The best combination of bacteriostatic agent and hormone concentration in subculture stage(bacteriostatic agent:50mg•L-1 ZP,150mL•L-1 Timentin,0.75mL•L-1 PPMTM;Medium:MS+2.5mg•L-1 6-BA+1.0mg•L-1 2,4-D+0.2mg•L-1 NAA),with pollution rate of 5.00%,proliferation coefficient of 5.43,vitrification rate of 1.75%;The best combination of culture medium bacteriostatic agent and hormone concentration in rooting culture stage(bacteriostatic agent:30mg•L-1 ZP,50mL•L-1 Timentin,0.75mL•L-1 PPMTM;Medium:1/2MS+1.0mg•L-1 IAA+0.5mg•L-1 NAA),the contamination rate was 10.00%,the rooting rate was 94.44%,the seedlings took root quickly,the stems were thick and the leaves were dark green.An open tissue culture and rapid propagation system for the cold-tolerant rose"2021-MH"was successfully established,which has the advantages of low contamination rate,high adventitious bud proliferation coefficient,low browning rate and vitrification rate,and favorable seedling growth performance.关键词
耐寒月季/开放式组培/快繁体系/抑菌剂/工厂化生产Key words
Cold-resistant rose/open tissue culture/rapid propagation system/bacteriostatic agent/industrialized seedling production分类
农业科技引用本文复制引用
张悦,苏雪娇,张爽爽,王秀峰,王健鹂,聂楚楚,李阿红..耐寒月季开放式组培快繁体系的建立[J].农业与技术,2026,46(1):11-18,8.基金项目
吉林省科技发展计划项目(项目编号:20230202106NC) （项目编号:20230202106NC）
