农业与技术2026，Vol.46Issue(1)：39-43,5.DOI:10.19754/j.nyyjs.20260130008
水翁叶多糖的提取及抗氧化活性分析
Extraction and antioxidant activity analysis of polysaccharides from Cleistocalyx operculatus leaves
摘要
Abstract
This experiment used Cleistocalyx operculatusleaves as the research material,optimized the extraction of polysaccharides from Cleistocalyx operculatus leaves using L9(34)orthogonal experimental method,and determined the antioxidant activity of the polysaccharide extract.The results showed that the optimal extraction conditions for polysaccharides were a solid-liquid ratio of 1∶10(g•mL-1),extraction time of 0.5 h,temperature of 80℃,and an extraction rate of 4.85%.The antioxidant results showed that polysaccharides from Cleistocalyx operculatus leaves have strong antioxidant activity,Its antioxidant capacity is positively correlated with polysaccharide concentration.Compared with the positive control Vc,the total reduction ability of polysaccharides is slightly stronger than Vc,and the clearance rate of DPPH•is comparable to Vc,but the clearance rate of hydroxyl radicals is weaker than Vc.关键词
水翁叶/多糖提取/正交试验/抗氧化活性Key words
Cleistocalyx operculatus leaf/polysaccharide extraction/orthogonal experiment/antioxidant activity分类
农业科技引用本文复制引用
黄丽华,陈钿薇,黄书画,张斌,徐敏凤,彭彬晓..水翁叶多糖的提取及抗氧化活性分析[J].农业与技术,2026,46(1):39-43,5.基金项目
肇庆市科技创新指导类项目(项目编号:241212094168550) （项目编号:241212094168550）
大学生创新创业训练计划项目(项目编号:X202510580090) （项目编号:X202510580090）
