农业与技术2026，Vol.46Issue(1)：50-53,4.DOI:10.19754/j.nyyjs.20260130010
小麦新品种系抗旱基因的KASP标记检测
KASP Marker Detection for Drought-Resistant Genes in New Wheat Lines
摘要
Abstract
To further clarify the distribution of drought tolerance genes in wheat new varieties(lines),two KASP markers(WH_K_1000070 and WH_K_1000038)closely linked to drought tolerance genes were used for molecular detection of TaDreb-B1 and 1-feh-w3 genes in 94 wheat new varieties(lines).The results showed that the detection rate of favorable alleles for marker WH_K_1000070(17.02%)was higher than that for marker WH_K_1000038(15.96%).A total of 3 haplotypes,1 heterozygous type,1 deletion type,and 5 allele combinations were detected in the tested materials.Among them,16 materials contained the favorable haplotype TaDreb-B1a,accounting for 17.02%;15 materials contained the favorable haplotype Westonia type,accounting for 15.96%;and 6 materials contained the optimal allele combination TaDreb-B1a+Westonia type,accounting for 6.38%.This study provides reference information for drought tolerance genetic breeding of wheat in the Huang-Huai Wheat Region.关键词
小麦/抗旱基因/KASP标记/分子检测Key words
wheat/drought tolerance genes/KASP markers/molecular detection分类
农业科技引用本文复制引用
蒋少威,白冬,李阳奇,王帅帅,王旭浩,谢焕鑫,邓跟望,朱统泉,陈杰..小麦新品种系抗旱基因的KASP标记检测[J].农业与技术,2026,46(1):50-53,4.基金项目
驻马店市农业科学院青年人才基金(项目编号:QNJJ2025003) （项目编号:QNJJ2025003）
驻马店市重点研发专项(项目编号:ZMDSZDYF2024008) （项目编号:ZMDSZDYF2024008）
河南省科技攻关项目(项目编号:242102111169,252102110287) （项目编号:242102111169,252102110287）
国家现代农业产业技术体系(项目编号:CARS-03) （项目编号:CARS-03）
