农业生物技术学报2026，Vol.34Issue(1)：129-138,10.DOI:10.3969/j.issn.1674-7968.2026.01.011
小麦光腥黑粉菌g5965蛋白的特性分析与功能研究
Characterization Analysis and Functional Study of Proteins g5965 of Tilletia foetida
摘要
Abstract
Wheat common bunt is one of the major wheat diseases which caused by Tilletia foetida,not only reducing wheat yield,but also deteriorating the quality of the wheat.To investigate the interaction mechanism between the common bunt fungus T.foetida and Triticum aestivum,a candidate effector protein g5965(GenBank No.XXN76190.1)belonging to the protein kinase superfamily was cloned via RT-PCR.The results of bioinformatics analysis revealed that the g5965 gene had a full length of 1 722 bp,encoded 573 amino acids,and contained a typical phosphotransferase domain.Its signal peptide secretion function had been verified through signal peptide secretion assays.Constructed the PGR107-g5965 fusion expression vector and transiently expressed it in the leaves of Nicotiana benthamiana via Agrobacterium-mediated transformation.The g5965 protein did not suppress the programmed cell death(PCD)response in tobacco cells induced by the pro-apoptotic protein BAX(Bcl-2 associated X protein).The green fluorescent protein(GFP)expression vector pBIN-g5965 was constructed and used to infect N.benthamiana leaves.The green fluorescence was observed at the cell membrane of tobacco cells under a laser scanning confocal microscope after 2～3 d.This study provides a certain theoretical basis for further elucidating the pathogenic mechanism of T.foetida against the wheat.关键词
小麦光腥黑粉菌/g5965/生物信息学分析/毒性验证/亚细胞定位Key words
Tilletia foetida/g5965/Bioinformatics analysis/Toxicity verification/Subcellular localization分类
农业科技引用本文复制引用
粟神强,文智伟,王昕,刘琦,高利..小麦光腥黑粉菌g5965蛋白的特性分析与功能研究[J].农业生物技术学报,2026,34(1):129-138,10.基金项目
国家重点研发计划(2024YFD1400200) （2024YFD1400200）
中国农业科学院南繁专项(YBXM2536) （YBXM2536）
中央级公益性科研院所基本科研业务费专项(Y2025YY01) （Y2025YY01）
新疆维吾尔自治区重大科技专项(2023A02009) （2023A02009）
新疆维吾尔自治区第一批"2+5"重点人才计划 （）
