农业生物技术学报2026，Vol.34Issue(2)：229-244,16.DOI:10.3969/j.issn.1674-7968.2026.02.001
家养动物抗病基因挖掘与新材料创制研究进展
Research Progress on Mining Disease Resistance Genes and Creating New Materials in Domestic Animals
摘要
Abstract
Disease resistance breeding in domestic animals is crucial for safeguarding the healthy development of the livestock industry and ensuring public health security.With the rapid advancement of high-throughput screening(HTS)technologies,the mining of disease resistance genes has entered a precision-and efficiency-oriented genomics era.This review systematically summarizes the principles and workflows involved in screening for disease resistance genes in domestic animals using core HTS technologies,including CRISPR-Cas9,RNA interference(RNAi),transcriptome analysis,protein interactomics,and epigenomics.It elaborates on the functional diversity of resistance genes identified through these techniques,classifying them into seven categories based on their mechanisms of action:Innate immune response,adaptive immune response,programmed cell death,viral adhesion and entry,intracellular trafficking,viral replication,and immune evasion.Furthermore,the article summarizes successful cases of creating novel disease-resistant materials and analyzes current challenges faced by HTS,such as off-target effects,efficiency problems,and data analysis complexity.Finally,the review prospects future directions involving interdisciplinary integration,technological optimization,and clinical translation,aiming to provide comprehensive theoretical references and technical pathways for disease resistance breeding research in domestic animals.关键词
高通量筛选/家养动物/抗病育种Key words
High-throughput screening/Domestic animals/Disease resistance breeding分类
农业科技引用本文复制引用
胡思宇,张然,赵要风..家养动物抗病基因挖掘与新材料创制研究进展[J].农业生物技术学报,2026,34(2):229-244,16.基金项目
国家重点研发计划(2023YFF1000900) （2023YFF1000900）
国家自然科学基金面上项目(32272839) （32272839）
