农业生物技术学报2026，Vol.34Issue(2)：245-253,9.DOI:10.3969/j.issn.1674-7968.2026.02.002
甘蓝型油菜BnaA05.nsLTP2基因克隆及表达模式分析
Gene Clone and Expression Pattern Analysis of BnaA05.nsLTP2 in Brassica napus
摘要
Abstract
Plant non-specific lipid transfer proteins(nsLTPs)are a type of small molecule soluble proteins that can transfer lipids between membranes and involve in multiple biotic and abiotic stress response.In our previous study,a non-specific lipid transfer protein,BnaA05.nsLTP2,that interact with key glycerol-3-phosphate acyltransferase,was identified by yeast two hybrid assay.In this study,the BnaA05.nsLTP2 gene was cloned from'Zhongshuang 11'variety of Brassica napus,and the length of coding sequence was 294 bp,encoding 97 amino acids,with a conserved domain of lipid transfer protein and 8 highly conserved cysteine residues.Phylogenetic analysis revealed that the sequence similarity between BnaA05.nsLTP2 and its homologous protein sequence in Brassica rapa reached 95.88%.In addition,the promoter region of BnaA05.nsLTP2 contained several cis-acting elements,including LTR(low-temperature response),ABRE(abscisic acid response),TCA-element(salicylic acid response),and so on.The pCAMBIA1305.1-35S-BnaA05.nsLTP2-GFP fusion expression vector was constructed,and the results of subcellular localization indicated that the protein was localized to cell membrane.The tissue-specific expression pattern analysis showed that the relative expression of the BnaA05.nsLTP2 gene was significantly higher in roots and stems than other tissues(P＜0.05).In addition,the BnaA05.nsLTP2 gene was induced by low temperature stress,osmotic and salt stress,suggesting that this gene might be involved in regulating the abiotic stress response mechanism of B.napus.This study provides theoretical basis for elucidating the biological function of BnaA05.nsLTP2 in B.napus.关键词
甘蓝型油菜/非特异性脂转移蛋白(nsLTPs)/基因克隆/亚细胞定位/表达模式Key words
Brasscia napus/Non-specific lipid transfer proteins(nsLTPs)/Gene cloning/Subcellular localization/Expression pattern分类
农业科技引用本文复制引用
彭姗,何李,张佳辉,吝瑞花,郑月萍,崔鹏,甘毅,刘宏波..甘蓝型油菜BnaA05.nsLTP2基因克隆及表达模式分析[J].农业生物技术学报,2026,34(2):245-253,9.基金项目
国家自然科学基金(32071929) （32071929）
国家重点研发计划(2022YFD1200403) （2022YFD1200403）
浙江省"三农九方"农业科技协作计划(2024SNJF008-1) （2024SNJF008-1）
浙江省"十四五"农业新品种选育重大科技专项(2021C02064-2-1) （2021C02064-2-1）
