热带亚热带植物学报2026，Vol.34Issue(1)：63-71,9.DOI:10.11926/jtsb.5003
罗浮柿EST-SSR分子标记开发和种群遗传多样性评价
Development of EST-SSR Molecular Markers for Diospyros morrisiana and Assessment of Population Genetic Diversity
摘要
Abstract
Based on the transcriptome data of Diospyros morrisiana seedlings,1620 pairs of polymorphic EST-SSR molecular markers were developed using CandiSSR software.The genetic diversity of 157 wild plants from 8 natural provenances was evaluated using 15 pairs of EST-SSR molecular markers randomly selected.The results showed that the transcriptome dataset contained 100 285 Unigene sequences with a total length of 98 738 602 bp;among them,34 121 SSR loci were identified,with a frequency of 34.02%,approximately one SSR locus every 2.89 kb.The 15 pairs of EST-SSR primers with good polymorphism had a polymorphism information content(PIC)ranging from 0.244 to 0.877,with an average of 0.623,and 12 loci were highly polymorphic(PIC:0.543-0.788).The observed heterozygosity of the 8 wild populations of D.morrisiana based on 15 pairs of EST-SSR primers ranged from 0.503 to 0.650,the expected heterozygosity ranged from 0.574 to 0.610,and the Shannon diversity index ranged from 1.072 to 1.245,indicating moderate genetic diversity.The majority of the genetic variation in the wild populations of D.morrisiana occurred within the populations(83.29%),and there was extensive gene flow among the populations,with a relatively close genetic relationship.These would provide technical support and scientific basis for the evaluation of germplasm resources,genetic diversity analysis,and molecular marker-assisted breeding of D.morrisiana.关键词
罗浮柿/引物开发/EST-SSR/遗传结构/遗传多样性Key words
Diospyros morrisiana/Primer development/EST-SSR/Genetic structure/Genetic diversity引用本文复制引用
汤瑞瑜,李想,黄钰琳,冯钰,张永华..罗浮柿EST-SSR分子标记开发和种群遗传多样性评价[J].热带亚热带植物学报,2026,34(1):63-71,9.基金项目
国家自然科学青年基金项目(31800309) （31800309）
温州市基础性公益科研项目(N20240014) （N20240014）
百山祖国家公园科学研究项目(2021KFLY06)资助 This work was supported by the National Natural Science Foundation of China(Grant No.31800309),the Project for Basic Research of Wenzhou(Grant No.N20240014),and the Project for Science Research of Baishanzu National Park(Grant No.2021KFLY06). （2021KFLY06）
