农业生物技术学报2026，Vol.34Issue(2)：283-294,12.DOI:10.3969/j.issn.1674-7968.2026.02.005
月季'绿萼'RcMADS2基因克隆及功能分析
Cloning and Functional Analysis of RcMADS2 Gene in Rosa chinensis'Viridiflora'
摘要
Abstract
The MADS-box gene family can determine floral morphogenesis and play an important role in floral organ development.Based on the previous transcriptome analysis of Rosa chinensis'Viridiflora',the MADS-box transcription factor-RcMADS2 related to floral organ variation was screened.In order to explore the biological function of this gene,this study cloned the RcMADS2,analyzed its biological information and expression pattern,and further verified its function by subcellular localization,heterologous overexpression and virus-induced gene silencing.The results showed that the CDS sequence of RcMADS2 was 762 bp,encoding 253 amino acids.It was predicted that the protein was an alkaline and unstable hydrophilic protein with highly conserved MADS-MEF2-like and K-box domains.The protein sequence had the highest similarity with the homologous sequence of R.rugosa.Phylogenetic tree analysis showed that RcMADS2 and E-class genes of Arabidopsis thaliana MADS-box family were divided into the same branch,and the genetic relationship was similar.By analyzing the expression pattern,it was found that the RcMADS2 gene had the highest expression level in the sepals of R.chinensis'Viridiflora',while it was basically not expressed in the leaves.Subcellular localization results showed that RcMADS2 was located in the nucleus,which was consistent with the prediction.Overexpression of RcMADS2 in A.thaliana showed that the number of sepals in A.thaliana positive plants changed and the growth potential of the plants decreased,and the expression of RcMADS2 in the plants increased significantly(P＜0.05).The qRT-PCR results showed that the expression of RcMADS2 in the silenced plants decreased,but no obvious phenotypic changes were found.This study preliminarily proved that the RcMADS2 gene of R.chinensis'Viridiflora'was an E-class gene,which was involved in the regulation of floral organ development,and provides relevant theories for the study of rose flower development in the later stage.关键词
月季'绿萼'/MADS-box/基因功能Key words
Rosa chinensis'Viridiflora'/MADS-box/Gene function分类
农业科技引用本文复制引用
张金凤,张华,宋利娜,张华丽,廖慧,杨一鹏,纪鹏..月季'绿萼'RcMADS2基因克隆及功能分析[J].农业生物技术学报,2026,34(2):283-294,12.基金项目
黑龙江省自然科学基金(LH2021C068) （LH2021C068）
绿色植物育种北京市重点实验室开放课题(YZZD202402) （YZZD202402）
