农业生物技术学报2026，Vol.34Issue(2)：295-305,11.DOI:10.3969/j.issn.1674-7968.2026.02.006
桂花OfLCYB基因上游转录因子的筛选和鉴定
Screening and Identification of Upstream Transcription Factors of OfLCYB Gene from Osmanthus fragrans
摘要
Abstract
Carotenoids are important pigments that determine the coloration of Osmanthus fragrans petals,and lycopene β-cyclase(LCYB)is a key enzyme in the carotenoid synthesis pathway of O.fragrans.In this study,O.fragrans cultivar'Yanhong Gui'was used as material,the bait vector of OfLCYB promoter and the yeast one-hybrid cDNA library of O.fragrans petals were constructed.The upstream transcription factor OfMYB308 interacting with OfLCYB promoter was screened by yeast one-hybrid.OfMYB308 had a typical EAR repressor motif and belonged to S4 subgroup of R2R3-MYB transcription factors.The expression level of OfLCYB was positively correlated with that of OfMYB308 gene,and both of them increased with flower opening.The results of yeast one-hybrid point-to-point verification and dual luciferase assay showed that OfMYB308 could bind to the OfLCYB promoter and significantly inhibit its transcriptional activity.This study provides theoretical basis for revealing the regulatory network of carotenoid synthesis in O.fragrans.关键词
桂花/类胡萝卜素/番茄红素β-环化酶(LCYB)/酵母单杂筛选Key words
Osmanthus fragrans/Carotenoids/Lycopene β-cyclase(LCYB)/Yeast one-hybrid screening分类
农业科技引用本文复制引用
许梦晗,卿洪生,钱婕妤,张超,付建新..桂花OfLCYB基因上游转录因子的筛选和鉴定[J].农业生物技术学报,2026,34(2):295-305,11.基金项目
国家自然科学基金(31501790) （31501790）
浙江省基础公益研究计划项目(LY19C160002) （LY19C160002）
