国际眼科杂志2026，Vol.26Issue(2)：202-207,6.DOI:10.3980/j.issn.1672-5123.2026.2.03
大豆异黄酮对激素性青光眼RGCs的保护作用研究
Protective effects of soy isoflavones on retinal ganglion cells in glucocorticoid-induced glaucoma
摘要
Abstract
·AIM:To investigate the neuroprotective effects of soy isoflavones(SI)against glucocorticoid-induced retinal ganglion cells(RGCs)injury in a rat model of acute ocular hypertension(AOH),and to explore the underlying mechanism involving the PI3K/Akt/Caspase-3 signaling pathway. ·METHODS:A total of 48 Sprague-Dawley(SD)rats(48 eyes)were randomly assigned to four groups(n=12 per group):a control group(received normal saline),an AOH control group,a low-dose SI intervention group[LSI+AOH,360 mg/(kg·d)],and a high-dose SI intervention group[HSI+AOH,540 mg/(kg·d)].The AOH,LSI+AOH,and HSI+AOH groups received subconjunctival injections of dexamethasone to induce AOH control group.From the 3 wk of modelling,SI was administered via intraperitoneal injection until 4 wk.Intraocular pressure(IOP)was monitored,RGC density was assessed,and the protein expression levels of PI3K/phosphorylated Akt(p-Akt)/Caspase-3,and key inflammatory cytokines were evaluated. ·RESULTS:IOP was significantly higher in the AOH control group compared to the control group(P<0.01).Both the LSI+AOH and HSI+AOH groups exhibited a marked reduction in IOP compared to the AOH control group(all P<0.01).RGC density was significantly lower in the AOH control group than in the control group(P<0.01),but was notably increased in the LSI+AOH group and the HSI+AOH group compared with the AOH control group(all P<0.01).Compared with the control group,the AOH control group demonstrated downregulated protein expression of PI3K and p-Akt,alongside upregulated expression of activated Caspase-3(all P<0.05).After intervention,the expression of PI3K and p-Akt was upregulated,while Caspase-3 was downregulated in the LSI+AOH group and the HSI+AOH group(all P<0.01). ·CONCLUSION:SI ameliorates glucocorticoid-induced RGCs injury,potentially by activating phosphorylated Akt(p-Akt)and subsequently inhibiting Caspase-3-mediated apoptosis.These neuroprotective effects appear to be dose-dependent.关键词
大豆异黄酮/激素性青光眼/PI3K/Akt信号通路/视网膜神经节细胞(RGCs)/细胞凋亡Key words
soy isoflavones/glucocorticoid-induced ocular hypertension/PI3K/Akt signaling pathway/retinal ganglion cells(RGCs)/apoptosis引用本文复制引用
曹慧,闫海波,张丽阳,杨华,李彦,王保君..大豆异黄酮对激素性青光眼RGCs的保护作用研究[J].国际眼科杂志,2026,26(2):202-207,6.基金项目
河南省医学科技攻关计划项目(No.LHGJ20230515) （No.LHGJ20230515）
新乡医学院博士科研基金资助项目(No.11359) Henan Medical Science and Technology Tackling Program Projects(No.LHGJ20230515) （No.11359）
Doctoral Scientific Research Foundation of Xinxiang Medical University(No.11359) （No.11359）
