生物医学工程研究2025，Vol.44Issue(6)：347-355,9.DOI:10.19529/j.cnki.1672-6278.2025.06.01
基于叉指电极的微血栓阻抗仿真与动态监测研究
Simulation and dynamic monitoring of microthrombus-related impedance changes based on interdigital electrode
摘要
Abstract
To alleviate the difficulties in the early identification of microthrombi and the lack of dynamic monitoring methods,we developed a high-sensitivity impedance monitoring system based on interdigital electrode(IDE)for label-free,real-time monitoring of microthrombus formation.Firstly,the finite element simulations were combined with in vitro thrombus formation experiments,thrombin was used to induce fibrinogen to form microthrombi,and the fluorescence intensity of fibrin was measured to quantify the formation process of microthrombi.Then,the influences of different red blood cell(RBC)concentrations,spatial distributions and thrombus sizes on the system impedance characteristics were comprehensively analyzed,and the real-time impedance changes and impedance frequen-cy responses during the dynamic development process of microthrombus were studied by using the IDE impedance monitoring system.Experimental results demonstrated that RBC aggregated on the IDE surface or bridging across positive and negative electrodes disrupted current conduction and increased system impedance.Low-frequency impedance was more sensitive to conductivity changes induced by microthrombi.High-frequency impedance exhibited only minor variations during thrombus formation.Real-time impedance monitoring further showed that the impedance-time curve followed the same trend as fibrin fluorescence intensity,characterized by a rapid initia-tion,gradual progression and plateau phase.The research provides an experimental approach for label-free,real-time monitoring of microthrombi,with potential applications in blood disease diagnosis and antithrombotic drug screening.关键词
叉指电极/微血栓/COMSOL/电阻抗/红细胞/动态监测Key words
Interdigital electrode/Microthrombus/COMSOL/Electrical impedance/Red blood cell/Dynamic monitoring分类
医药卫生引用本文复制引用
常丽芸,张益之,杨雅敏..基于叉指电极的微血栓阻抗仿真与动态监测研究[J].生物医学工程研究,2025,44(6):347-355,9.基金项目
中央高校基本科研业务费(NZ2024034). （NZ2024034）
