生物医学工程研究2025，Vol.44Issue(6)：395-402,8.DOI:10.19529/j.cnki.1672-6278.2025.06.07
基于光电倍增管的四通道小动物在体荧光监测系统
Four-channel in vivo fluorescence monitoring system using photomultiplier tube
李伟 1李怡燃 1陈超凡 1陶玲 1钱志余 1晋晓飞 1李韪韬1
作者信息
- 1. 南京航空航天大学 自动化学院,南京 211106
- 折叠
摘要
Abstract
Aiming at the problems that the collection process of pharmacokinetic data for ex vivo monitoring is easily affected by in-sufficient sample size,delayed data collection and significant differences among individuals,we developed a four-channel in vivo fluo-rescence monitoring system with real-time data acquisition by integrating hardware components and the LabView platform.Based on the principle of laser-induced fluorescence,this system utilized the unique gain mechanism of photomultiplier tube(PMT)to amplify weak optical signals.The sensitivity and effectiveness of the system were confirmed by one-way analysis of variance(P<0.05)on the back-ground light and the number of fluorescent photons in 0.25 μg/mL fluorescein isothiocyanate(FITC)solution.In vivo experiment fur-ther demonstrated that this system could not only effectively achieve real-time monitoring of fluorescence signals in major metabolic or-gans such as the kidneys,but also minimized data errors caused by ex vivo experiment and improve data accuracy.It can provide more comprehensive data for subsequent research on drug efficacy and therapeutic mechanisms.关键词
光电倍增管/荧光监测/在体监测/四通道/药代动力学Key words
Photomultiplier tube/Fluorescence monitoring/In vivo monitoring/Four-channels/Pharmacokinetics分类
医药卫生引用本文复制引用
李伟,李怡燃,陈超凡,陶玲,钱志余,晋晓飞,李韪韬..基于光电倍增管的四通道小动物在体荧光监测系统[J].生物医学工程研究,2025,44(6):395-402,8.
