南京中医药大学学报2026，Vol.42Issue(1)：53-64,12.DOI:10.14148/j.issn.1672-0482.2026.0053
"肺与大肠相表里"的全生命周期代谢全景图谱:基于大鼠肺组织、血清与粪便样本的跨器官代谢调控研究
A Whole-Life Cycle Metabolic Landscape of"the Lung and Large Intestine Being Internally-Externally Related":Cross-Organ Metabolic Regulation Based on Rat Lung,Plasma,and Fecal Profiles
摘要
Abstract
OBJECTIVE Based on the traditional Chinese medicine(TCM)theory of"the lung and large intestine being internally-externally related",to systematically characterize metabolic changes in the lung,serum,and feces of rats at different develop-mental stages and analyze their intrinsic connections employing non-targeted metabolomics,aiming to elucidate the material basis of the"gut-blood-lung"metabolic axis and provide experimental evidence for metabolic intervention and TCM-based treatment of pediat-ric respiratory diseases.METHODS Gas chromatography-mass spectrometry(GC-MS)was used to detect metabolites in lung tissue,serum,and fecal samples from rats aged 3,7,12,24,56 and 70 weeks.Principal component analysis(PCA)and variable importance in projection(VIP)screening were conducted to identify key metabolites.Common metabolites among the three sample types and differen-tial metabolites between juvenile(3-week-old)and adult(7-week-old)rats were further analyzed.RESULTS High-VIP metabolites in lung tissue and serum included β-glycerophosphate,glucose-1-phosphate,maltose,xanthine,hypoxanthine,lauric acid,and sorbi-tol,while those in feces were mainly 17α,20α-dihydroxy-4-pregnene-3-one,4-hydroxyphenylacetic acid,and putrescine.Shared me-tabolites among all sample types included lactate,β-hydroxybutyrate,glycine,and glutamine.Significant differences in metabolic pro-files were observed between juvenile and adult rats across lung,serum,and fecal samples.KEGG pathway enrichment analysis re-vealed that taurine and hypotaurine metabolism,alanine,aspartate and glutamate metabolism,and arginine and proline metabolism were highly impacted in juvenile lung and serum,while alanine,aspartate and glutamate metabolism,starch and sucrose metabolism,and arginine biosynthesis were predominant in feces.CONCLUSION The metabolic characteristics of the lung,blood,and intestine exhibit distinct age-dependent patterns,with amino acid and energy metabolism being more active during the juvenile stage,reflecting the high metabolic demands of tissue development and immune homeostasis.These dynamic changes in the"gut-blood-lung"meta-bolic axis may constitute the material basis of the TCM theory of"the lung and large intestine being internally-externally related",offer-ing new scientific insights for TCM-based diagnosis and metabolic intervention in pediatric respiratory diseases.关键词
肺与大肠相表里/非靶向代谢组学/肺/血清/粪便/氨基酸代谢Key words
the lung and large intestine being internally-externally related/non-targeted metabolomics/lung/serum/feces/amino acid metabolism分类
医药卫生引用本文复制引用
高雅楠,陈涛,杨斌,王璇,时晨,许伟辰,罗子宸,徐建亚,单进军.."肺与大肠相表里"的全生命周期代谢全景图谱:基于大鼠肺组织、血清与粪便样本的跨器官代谢调控研究[J].南京中医药大学学报,2026,42(1):53-64,12.基金项目
国家自然科学基金面上项目(82174098) （82174098）
国家重点研发计划项目(2024YFC3505900,2024YFC3505904) （2024YFC3505900,2024YFC3505904）
南京中医药大学中药制药过程控制与智能制造技术全国重点实验室创新项目(NZYSKL240106) （NZYSKL240106）
2025年度江苏省中医流派研究院重点课题(LPZD2025012) （LPZD2025012）
2024年度南京中医药大学科研平台人才专项 （）
2025年度江苏省研究生科研创新计划(KYCX25_2368) （KYCX25_2368）
