南京中医药大学学报2026，Vol.42Issue(1)：79-89,11.DOI:10.14148/j.issn.1672-0482.2026.0079
降气平哮方调控CLCA1/Ca2+/SNARE信号改善哮喘气道黏液高分泌的机制研究
Mechanistic Study of Jiangqi Pingxiao Formula in Ameliorating Mucus Hypersecretion in Asthmatic Air-ways via Regulating CLCA1/Ca2+/SNARE Pathway
摘要
Abstract
OBJECTIVE To investigate the mechanism by which Jiangqi Pingxiao Formula(JQPXF)regulates Ca2+and mem-brane fusion to attenuate airway mucus hypersecretion in asthma.METHODS An ovalbumin-induced asthma model was established in mice.The experimental groups included the normal control group,the asthma model group,JQPXF low-(10.0 g·kg⁻¹),medium-(20.0 g·kg⁻¹),and high-dose(30.0 g·kg⁻¹)groups,the dexamethasone group(1.0 mg·kg⁻¹),and the ambroxol hydrochloride group(8.0 mg·kg⁻¹).During the model construction period,the status of mice were observed through behavioral observation,and lung patho-logical status were evaluated by HE and PAS staining.The levels of inflammatory cytokines interleukin(IL)-4 and IL-13 in mouse lungs were detected using the ELISA method.qPCR was used to detect mucin mRNA expression,calcium ion(Ca²⁺)concentration in mouse lung tissue was measured by colorimetric assays,and immunohistochemistry and Western blot analyses were used to assess the expression of calcium-activated chloride channel modulator 1(CLCA1)and membrane fusion-related proteins.RESULTS Com-pared with the normal group,the model group exhibited significantly increased airway inflammation scores,mucus secretion,IL-13 and IL-4 levels,calcium ion concentration,and expression of CLCA1;synaptophysin-associated protein 23(SNAP23),soluble N-ethylmaleimide-sensitive receptor protein(SNARE),synaptotagmin(STX3),vesicle-associated membrane protein 8(VAMP8),and vesicle-associated membrane protein 2(VAMP2)were significantly elevated(P<0.05,P<0.01,P<0.001).Compared with the model group,JQPXF effectively improved various clinical signs in asthmatic mice,significantly alleviating airway inflammation and mucus se-cretion(P<0.05,P<0.001);reduced IL-4 and IL-13 levels in lung tissue(P<0.001);suppressed the overexpression of mucin genes(P<0.05,P<0.01,P<0.001);decreased intracellular Ca²⁺ concentration(P<0.05,P<0.001);and downregulated the expression of key proteins in the membrane fusion pathway,including CLCA1,SNAP23,SNARE,STX3,VAMP8 and VAMP2.CONCLUSION JQPXF may ameliorate mucus hypersecretion in asthmatic airways by suppressing the CLCA1/Ca²⁺/SNARE signaling pathway,provid-ing a novel molecular mechanism target for JQPXF therapy.关键词
哮喘/降气平哮方/气道黏液高分泌/CLCA1/Ca2+/膜融合Key words
asthma/Jiangqi Pingxiao Formula/airway mucus hypersecretion/CLCA1/Ca2+/membrane fusion分类
医药卫生引用本文复制引用
石玲玲,严花,吴嘉宝,单祎文,董盈妹,赵霞..降气平哮方调控CLCA1/Ca2+/SNARE信号改善哮喘气道黏液高分泌的机制研究[J].南京中医药大学学报,2026,42(1):79-89,11.基金项目
国家重点研发计划"中医药现代化"重点专项(2024YFC3505900,2024YFC3505902) （2024YFC3505900,2024YFC3505902）
江苏省高等学校基础科学(自然科学)研究项目资助(24KJB360011) （自然科学）
江苏省中医药领军人才培养项目(SLJ0320) （SLJ0320）
国家自然科学基金青年科学基金项目(82505670) （82505670）
江苏高校优势学科建设工程(PAPD) （PAPD）
