色谱2026，Vol.44Issue(1)：17-29,13.DOI:10.3724/SP.J.1123.2025.04007
细胞印迹技术在生物医学领域的应用进展
Advances in application of cell imprinting technology in biomedical field
摘要
Abstract
Cell imprinting technology,as a key technique in the field of cell-specific recognition,has become an important cornerstone of modern biomedical research.It relies on its precise rec-ognition capability for biomarkers such as cell surface antigens and receptors.This technology is not only widely applied in basic fields,including disease marker detection and research on cell function and behavioral mechanisms,but also plays a crucial role in frontier applications like rare cell isolation and targeted drug development.It continuously promotes the innovative development of life science research.Although significant progress has been made in this technology,it still faces several urgent challenges.These specifically include optimization of the imprinting process(to enhance anti-interference ability and fidelity)and development of new methods adapted to complex biological environments.In response to these issues,researchers have achieved breakthrough progress in multiple fields through innovative material design and process optimization recently.First,in rare cell capture,this technology has significantly improved the separation efficiency of key rare cells in complex samples,providing an efficient solution for early cancer diagnosis and blood disease research.Second,in the field of cell culture,its unique imprinted surface can precisely regulate the expression of cell adhesion proteins to optimize the in vitro culture microenvironment.Finally,in biosensing,sensors developed based on this technology have demonstrated excellent sensitivity and specificity,opening up new avenues for disease monitoring.This paper systematically reviews the research status and development trends of cell imprinting technology,deeply analyzes its technical bottlenecks and breakthrough directions.The aim is to provide a comprehensive theoretical reference for related research and further promote the innovative application and leapfrog development of this technology in the biomedical field.关键词
细胞印迹/细胞捕获/细胞识别与分选/细胞培养/生物传感器Key words
cell imprinting/cell capture/cell recognition and sorting/cell culture/biosensor分类
化学化工引用本文复制引用
赵鑫淼,张志远,孙文静,卿光焱..细胞印迹技术在生物医学领域的应用进展[J].色谱,2026,44(1):17-29,13.基金项目
国家重点研发计划(2022YFC3400800) （2022YFC3400800）
国家自然科学基金(21922411,22174138) （21922411,22174138）
中国科学院大连化学物理研究所创新基金(I202243,I202229) （I202243,I202229）
大连市杰出青年科技人才项目(2020RJ01). National Key Research and Development Program of China(No.2022YFC3400800) （2020RJ01）
National Natural Science Foundation of China(Nos.21922411,22174138) （Nos.21922411,22174138）
DICP Innovation Funding(Nos.I202243,I202229) （Nos.I202243,I202229）
Dalian Outstanding Young Scientific Talent(No.2020RJ01). （No.2020RJ01）
