日用化学工业（中英文）2026，Vol.56Issue(1)：55-64,10.DOI:10.3969/j.issn.2097-2806.2026.01.007
基于网络药理学和细胞实验探究木芙蓉叶抗炎的物质基础及作用机制
Exploring the material basis and mechanisms of the action of Hibiscus mutabilis L.for its anti-inflammatory effects based on network pharmacology and cell experiments
摘要
Abstract
To explore the material basis and mechanisms of the anti-inflammatory effects of Hibiscus mutabilis L..The active ingredients and potential targets of Hibiscus mutabilis L.were obtained through the literature review and SwissADME platform.Genes related to the inflammation were collected using Genecards and OMIM databases,and the intersection genes were submitted on STRING and DAVID websites.Then,the protein interaction network(PPI),gene ontology(GO)and pathway(KEGG)were analyzed.Cytoscape 3.7.2 software was used to construct the"Hibiscus mutabilis L.-active ingredient-target-inflammation"network diagram,and AutoDockTools-1.5.6 software was used for the molecular docking verification.The anti-inflammatory effect of Hibiscus mutabilis L.active ingredient was verified by the RAW264.7 inflammatory cell model.The results showed that 11 active components and 94 potential targets,1029 inflammatory targets and 24 intersection targets were obtained from Hibiscus mutabilis L..The key anti-inflammatory active ingredients of Hibiscus mutabilis L.are quercetin,apigenin and luteolin.Its action pathway is mainly related to NF-κB,cancer pathway and TNF signaling pathway.Cell experiments showed that total flavonoids of Hibiscus mutabilis L.could effectively inhibit the expression of tumor necrosis factor(TNF-α),interleukin 8(IL-8)and epidermal growth factor receptor(EGFR)in LPS-induced RAW 264.7 inflammatory cells.It also down-regulates the phosphorylation of human nuclear factor ĸB inhibitory protein α(IĸBα)and NF-κB p65 subunit protein(p65).Overall,the anti-inflammatory effect of Hibiscus mutabilis L.is related to many active components,many signal pathways and targets,which provides a theoretical basis for its further development and application.关键词
木芙蓉叶/炎症/网络药理学/分子对接/细胞验证Key words
Hibiscus mutabilis L./inflammation/network pharmacology/molecular docking/cell validation分类
化学化工引用本文复制引用
谌文元,陈晓兰,万静,邓琴,高雍..基于网络药理学和细胞实验探究木芙蓉叶抗炎的物质基础及作用机制[J].日用化学工业（中英文）,2026,56(1):55-64,10.基金项目
贵州省高层次创新型人才项目(黔科合平台人才-GCC[2023]037) （黔科合平台人才-GCC[2023]037）
国家苗药工程技术研究中心能力提升(黔科合中引地[2023]006) （黔科合中引地[2023]006）
贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2025-066) （QZYY-2025-066）
毕节市科技计划项目(毕科合(2024)15) （毕科合(2024）
