沈阳医学院学报2026，Vol.28Issue(1)：9-17,9.DOI:10.16753/j.cnki.1008-2344.2026.01.003
BAP31对急性炎症中M1型巨噬细胞的影响及其机制分析
The effect and mechanism analysis of BAP31 on M1 macrophages in acute inflammation
摘要
Abstract
Objective:To investigate the regulatory effect of B cell associated receptor protein 31(BAP31)on M1 macrophages in acute inflammation.Methods:Lyz2Cre-BAP31flox/flox and BAP31flox/flox mice were intraperitoneally injected with lipopolysaccharide(LPS)to establish an acute inflammation mouse model,and the effects of LPS on body weight and colon length of each group were evaluated.Bone marrow cells were extracted from the femurs of BAP31flox/flox and Lyz2Cre-BAP31flox/flox mice for in vitro experiments.After induction into macrophages using 10%L929 mouse fibroblast supernatant,the expression of genes and proteins related to macrophage polarization was detected by RT-qPCR and Western blot.Results:In the acute inflammation model,compared with Lyz2Cre-BAP31flox/flox mice injected with PBS,Lyz2Cre-BAP31flox/flox mice injected with LPS for 72 h showed decreased body weight and significantly shortened colon length.There was a large amount of inflammatory cell infiltration and significant glandular atrophy in the intestinal mucosa.In vitro experiments showed that bone marrow-derived macrophages from Lyz2Cre-BAP31flox/flox mice,compared to those from BAP31flox/flox mice,showed significantly increased expression of M1 macrophage-related genes after stimulation with LPS,IFNγ,or LPS+IFNγ,while M2 macrophage-related genes was significantly decreased.Western blot results showed that BAP31 knockdown upregulated the protein expression levels of p-STAT1 and p-ERK.Conclusion:Knockdown of BAP31 affects M1 macrophage activation by regulating the phosphorylation levels of STAT1 and ERK,providing a new target for the prevention and treatment of acute inflammation.关键词
B细胞相关受体蛋白31/巨噬细胞/急性炎症Key words
BAP31/macrophage/acute inflammation分类
医药卫生引用本文复制引用
贺娟,韩竹军,赵千玉,张珉,王旭光,薄威,王晓开,张忠,王晓宇..BAP31对急性炎症中M1型巨噬细胞的影响及其机制分析[J].沈阳医学院学报,2026,28(1):9-17,9.基金项目
国家自然科学基金青年基金(No.82400695) （No.82400695）
辽宁省联合基金项目面上项目(No.2023-MSLH-298) （No.2023-MSLH-298）
辽宁省教育厅课题(No.LJ212410164016) （No.LJ212410164016）
辽宁省科技计划联合计划博士科研启动项目(No.2024-BSLH-295) （No.2024-BSLH-295）
