沈阳医学院学报2026，Vol.28Issue(1)：30-37,8.DOI:10.16753/j.cnki.1008-2344.2026.01.006
miR-30a-5p通过调控CTHRC1抑制甲状腺未分化癌进展
miR-30a-5p inhibits the progression of anaplastic thyroid carcinoma by regulating CTHRC1
摘要
Abstract
Objective:To investigate the relationship of the expression of collagen triple helix repeat containing-1(CTHRC1)in thyroid cancer with patient prognosis and immune cell infiltration,and to further explore the effect of CTHRC1 on the proliferation,migration,invasion and apoptosis of anaplastic thyroid carcinoma(ATC)cells in vitro and the role of miR-30a-5p in this process.Methods:The expression of CTHRC1 in thyroid carcinoma and its relationship with survival,clinicopathological features and immune cell infiltration were analyzed based on The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO).RT-qPCR and Western blot were used to detect CTHRC1 mRNA and protein expression.CCK-8,Transwell assays,and flow cytometry were employed to detect the proliferation,migration,invasion,and apoptosis of ATC cells.The ENCORI database was used to screen for the upstream regulator of CTHRC1,miR-30a-5p,and the expression correlation between them in thyroid cancer was analyzed.RT-qPCR was used to detect the effect of miR-30a-5p overexpression on CTHRC1 mRNA expression in ATC cells,and a dual-luciferase reporter gene assay was performed to validate their binding.Results:TCGA database analysis showed that the expression of CTHRC1 mRNA was up-regulated in thyroid carcinoma and was related to poor prognosis.Immune infiltration analysis showed that CTHRC1 expression in thyroid carcinoma was related to various immune cell populations,including T cells,B cells,cytotoxic cells,NK cells and dendritic cells.GEO database analysis showed that the expression of CTHRC1 was up-regulated in ATC tissues,and RT-qPCR confirmed that CTHRC1 mRNA was up-regulated in several ATC cells.Knockdown of CTHRC1 inhibited the proliferation,migration and invasion of ATC cells and promotd apoptosis.The dual-luciferase reporter gene assay verified the combination of CTHRC1 and miR-30a-5p.Overexpression of miR-30a-5p reduced the expression of CTHRC1 mRNA in ATC cells.The expression of miR-30a-5p and CTHRC1 showed a significant negative correlation in thyroid cancer.Inhibition of miR-30a-5p reversed the suppressive effect of CTHRC1 knockdown on the proliferation,migration and invasion of ATC cells,as well as the promotion of apoptosis.Conclusion:CTHRC1 may be an important prognostic biomarker for ATC,and miR-30a-5p inhibits the malignant biological behavior of ATC cells by regulating CTHRC1.关键词
甲状腺未分化癌/CTHRC1/miR-30a-5p/细胞迁移和侵袭/细胞凋亡Key words
anaplastic thyroid carcinoma/CTHRC1/miR-30a-5p/cell migration and invasion/cell apoptosis分类
医药卫生引用本文复制引用
陈晶晶,俞建华,张凤霞,孙成林,康永生..miR-30a-5p通过调控CTHRC1抑制甲状腺未分化癌进展[J].沈阳医学院学报,2026,28(1):30-37,8.基金项目
辽宁省科学技术计划重大科研项目(No.2022JH2/101300035) （No.2022JH2/101300035）
沈阳市科学技术计划项目基金(No.21-173-9-18) （No.21-173-9-18）
