实用医学杂志2026，Vol.42Issue(3)：406-415,10.DOI:10.3969/j.issn.1006-5725.2026.03.007
祛湿活血方调控AMPK/SREBP1c信号通路对HepG2脂肪肝细胞生长的影响
The effect of the Qushi Huoxue decoction on the AMPK/SREBP1c signaling pathway in free fatty acid-induced HepG2 cells
摘要
Abstract
Objective To investigate the effect of Qushi Huoxue decoction(QSHXF)on free fatty acid(FFA)-induced HepG2 cells and its underlying mechanism.Methods In vitro cell experiments were conducted.First,the CCK-8 assay was used to determine the effects of different concentrations of QSHXF on HepG2 cells viability to select appropriate concentrations for subsequent experiments.A fatty liver cell model was then established by treating HepG2 cells with a mixture of 250 μmol/L sodium palmitate and 500 μmol/L sodium oleate for 24 h,followed by intervention with different concentrations of QSHXF-containing serum and AMPK inhibitor for another 24 h.Intracellular lipid droplet accumulation was observed using Oil Red O staining and lipid fluorescence staining.The levels of total cholesterol(TC)and triglycerides(TG)were measured using the GPO-PAP enzymatic method.The TBA method and hydroxylamine method were used to detect malondialdehyde(MDA)and superoxide dis-mutase(SOD),respectively,while ELISA was used to detect tumor necrosis factor-α(TNF-α)and interleukin 1β(IL-1β).Furthermore,qRT-PCR,Western blot,and immunofluorescence staining were performed to detect the expression of lipid metabolism-related genes and proteins.Results QSHXF significantly improved the lipid deposition,oxidative stress and inflammatory response of FFA-induced HepG2 cells,markedly reduce the content of TC,TG,MDA,TNF-α and IL-1β in cells and increase the activity of SOD(P<0.01).Meanwhile,it increased the mRNA expression of AMPK and the protein ratio of p-AMPK/AMPK,while decreasing the mRNA and protein levels of SREBP1c,FASN,ACC1,and SCD1(all P<0.05).Conclusion QSHXF significantly improved lipid metabolism and alleviated oxidative stress and inflammation in the MASLD cell model,and its mechanism may be associated with the activation of the AMPK/SREBP1c signaling pathway.关键词
祛湿活血方/代谢功能障碍相关脂肪性肝病/AMPK/SREBP1c信号通路/HepG2细胞Key words
Qushi Huoxue decoction/non-alcoholic fatty liver disease/AMPK/SREBP1c signaling pathway/HepG2 cells分类
医药卫生引用本文复制引用
谭伟强,刘丽,刘旭东,魏业煌,龚晓倩,王蓉蓉..祛湿活血方调控AMPK/SREBP1c信号通路对HepG2脂肪肝细胞生长的影响[J].实用医学杂志,2026,42(3):406-415,10.基金项目
国家自然科学基金项目(编号:82560866,82160837) （编号:82560866,82160837）
全国中医临床优秀人才研修项目(编号:国中医药人教函[2022]1号) （编号:国中医药人教函[2022]1号）
广西研究生教育创新计划项目(编号:YCBZ2025189) （编号:YCBZ2025189）
2022年广西(青年)岐黄学者培养项目 （青年）
