西北农业学报2026，Vol.35Issue(2)：260-274,15.DOI:10.7606/j.issn.1004-1389.2026.02.007
甘蓝型油菜EMS突变体库特异脂肪酸构成材料苗期耐寒性评价及其基因挖掘
Evaluation of Seedling Cold Tolerance and Gene Mining in Brassica napus Germplasm with Specific Fatty Acid Profiles from an EMS Mutant Library
摘要
Abstract
Rapeseed is an important source of edible oil and forage protein in China.Improving seed oil content,optimizing fatty acid composition,and enhancing cold tolerance are crucial goals in rapeseed breeding.This study utilized an Ethylmethanesulfonate(EMS)mutant library of Brassica napus'Zhongshuang 11'constructed with 1%EMS mutagenesis.After two consecutive self-crosses com-bined with quality screening,259 germplasm lines with specific fatty acid profile were obtained.Subse-quently,19 M4 materials that survived under the prolonged low temperatures during the 2022-2023 overwintering period and produced sufficient seeds were evaluated for cold tolerance.The study found that two cold-tolerant germplasms exhibited enhanced enzymatic scavenging capabilities compared to two cold-sensitive germplasms,resulting in reduced levels of reactive oxygen species and malondialde-hyde.Further transcriptome sequencing of the cold-tolerant germplasm 3Y1210-4#1 and the cold-sen-sitive germplasm 3Y1353-2#4 only identified 2 609 low-temperature-responsive differentially ex-pressed genes(DEGs).GO enrichment analysis of these DEGs revealed that they are broadly involved in cold response,fatty acid synthesis,hormone regulation,oxidative stress,and osmotic stress.关键词
甘蓝型油菜/EMS突变体库/特异脂肪酸构成/耐寒/转录组Key words
Brassica napus/EMS mutant library/Specific fatty acids profile/Cold tolerance/Tran-scriptomics引用本文复制引用
何铭渊,邵亚楠,刘华,崔晓辉,马芸芸,马少杰,刘子金,陈明训,郭媛..甘蓝型油菜EMS突变体库特异脂肪酸构成材料苗期耐寒性评价及其基因挖掘[J].西北农业学报,2026,35(2):260-274,15.基金项目
国家重点研发计划(2024YFD1200400) （2024YFD1200400）
国家科技创新2030(2022ZD04010) （2022ZD04010）
陕西省科技创新团队(2024RS-CXTD-69). National Key Research and Development Program(No.2024YFD1200400) （2024RS-CXTD-69）
the Sci-Tech Innovation 2030(No.2022ZD04010) （No.2022ZD04010）
the Scientific and Technological Innovation Team of Shaanxi Province(No.2024RS-CXTD-69). （No.2024RS-CXTD-69）
