应用生态学报2025，Vol.36Issue(12)：3709-3717,9.DOI:10.13287/j.1001-9332.202512.011
不同有机物料深混对白浆土土壤胞外酶活性及化学计量特征的影响
Effects of deep ploughing incorporation of organic materials to field on the extracellular enzyme activities and stoichiometric characteristics in albic soil
摘要
Abstract
Deep incorporation of organic materials is an effective practice for soil improvement.We conducted a two-year experiment in typical albic soil with five treatments:conventional tillage(CT),deep tillage(DT),straw deep incorporation(DS),organic fertilizer deep incorporation(DM),and straw-organic fertilizer deep incorpora-tion(DSM).We investigated the effects of deep incorporation of different organic materials on microbial biomass,extracellular enzyme activities,and their stoichiometric ratios in albic soil.The results showed that deep incorpora-tion of organic materials(DS,DM,and DSM)significantly increased microbial biomass carbon(MBC),microbial biomass nitrogen(MBN),and microbial biomass phosphorus(MBP)in both the 0-15 cm and 15-35 cm soil lay-ers,with the DSM treatment showing the strongest effect.MBC,MBN,and MBP under DSM increased by 72.8％,61.6％,and 147.7％in the 0-15 cm soil layer,and 78.7％,136.7％,and 248.4％in the 15-35 cm soil layer,respectively.Extracellular enzyme activities decreased significantly with increasing soil depth.The addition of organ-ic materials significantly enhanced enzyme activities in each soil layer with DSM again exhibiting the largest promo-tion.The activities of C-acquiring enzyme(β-1,4-glucosidase),N-acquiring enzymes(β-1,4-N-acetylglucosamin-idase and leucine aminopeptidase),and P-acquiring enzyme(acid phosphatase)under DSM increased by 11.6％,65.3％,and 50.4％in the 0-15 cm layer,and 68.9％,37.4％,and 31.1％in the 15-35 cm layer compared to CT,respectively.The enzyme-based vector model revealed that soil microbial communities across all layers of the albic soil were generally co-limited by carbon and phosphorus.Deep incorporation of organic materials significantly alleviated the nutrient limitation.Correlation analysis and random forest modeling further indicated that soil extracel-lular enzyme activities were regulated by MBC,MBP and MBN.Deep incorporation of organic materials could en-hance microbial and enzymatic activities in albic soil and promote nutrient cycling,with the combined application of straw and organic fertilizer having the highest improvement.关键词
白浆土/深耕/有机物料还田/土壤胞外酶活性/土壤养分Key words
albic soil/deep ploughing/organic material return to field/soil extracellular enzyme activity/soil nu-trient引用本文复制引用
高瑞敏,严君,韩晓增,陈旭,邹文秀,陆欣春,贺娟妮..不同有机物料深混对白浆土土壤胞外酶活性及化学计量特征的影响[J].应用生态学报,2025,36(12):3709-3717,9.基金项目
本文由国家重点研发计划项目(2022YFD1500802)和中国科学院战略性先导科技专项A类(XDA28070000)资助. （2022YFD1500802）
