医学信息2026，Vol.39Issue(1)：45-48,4.DOI:10.3969/j.issn.1006-1959.2026.01.007
绣球防风提取物对LPS诱导RAW264.7细胞NO表达研究
Study on the Effect of Leucas Ciliata Benth.Extract on NO Expression in LPS-induced RAW264.7 Cells
摘要
Abstract
Objective To investigate the inhibitory effect of the traditional Chinese medicine Leucas ciliata Benth.extract on lipopolysaccharide(LPS)-induced nitric oxide(NO)expression in RAW264.7 cells and its molecular mechanism.Methods The impact of different concentrations of Leucas ciliata Benth.extract on RAW264.7 cell viability was assessed using the CCK-8 assay to determine the safe and effective concentration range.The Griess method was used to measure NO release levels in cell culture supernatants,reflecting the regulatory effect of the Leucas ciliata Benth.extract on LPS-induced NO expression in RAW264.7 cells.Results At concentrations up to 200 μg/ml,Leucas ciliata Benth.extract showed no significant effect on RAW264.7 cell viability(P>0.05).Concentrations of 25 μg/ml and higher significantly inhibited NO release in LPS-stimulated RAW264.7 cells(P<0.001),exhibiting a concentration-dependent effect.Conclusion Leucas ciliata Benth.extract effectively inhibits LPS-induced excessive NO expression in RAW264.7 cells,with the inhibitory effect closely correlated with the concentration of the extract.关键词
绣球防风/RAW264.7细胞/脂多糖/一氧化氮Key words
Leucas ciliata Benth./RAW264.7 cells/Lipopolysaccharide/Nitric oxide分类
医药卫生引用本文复制引用
赵有亮,付晓云..绣球防风提取物对LPS诱导RAW264.7细胞NO表达研究[J].医学信息,2026,39(1):45-48,4.基金项目
云南省教育厅科学研究基金项目(编号:2024J1641) （编号:2024J1641）
