空军军医大学学报2026，Vol.47Issue(1)：11-19,9.DOI:10.13276/j.issn.2097-1656.2026.01.002
电磁悬吊间歇性失重与体液重分布耦合作用诱导小鼠颈总动脉重塑及干预靶点筛选
Electromagnetic suspension-induced coupling effects of intermittent weightlessness and cephalad fluid shift on common carotid artery remodeling and therapeutic target screening in mice
摘要
Abstract
Objective To systematically evaluate the structural remodeling effect of the common carotid artery induced by a self-developed electromagnetic suspension device in mice and to screen key molecular targets.Methods An intermittent cephalad fluid shift,low mechanical load,and weightlessness coupled mouse model was established using the electromagnetic suspension device.The carotid intima-media thickness(c-IMT),collagen deposition,and elastic fiber structure of the common carotid artery were assessed by HE,Masson,and EVG staining.Differentially expressed genes and enriched pathways were analyzed using transcriptome sequencing combined with bioinformatics.Results Compared with the control group,mice in the coupled effects(CE)group showed significantly increased bilateral c-IMT,elevated collagen fiber area percentage,decreased elastic fiber width,and an increased number of elastic fiber breakpoints.Transcriptome sequencing identified 6 114 differentially expressed genes in the CE group's common carotid arteries.GO and KEGG analyses indicated these genes were significantly enriched in processes such as"extracellular matrix organization"and the"Ras/MAPK signaling pathway".Comparison with transcriptomic data from human aortic smooth muscle cells under real microgravity conditions revealed 272 shared differentially expressed genes.Conclusion The electromagnetic suspension device can simulate fluid redistribution and partially reproduce vascular structural remodeling associated with weightlessness.A molecular regulatory network centered on MMP15/24,WNT6,FGF1,TIMP3,FBLN1/5,and TGM2 is preliminarily revealed,providing a new experimental platform for mechanistic investigation and the development of corresponding protective strategies.关键词
失重/小鼠/颈总动脉/血管重塑/细胞外基质/基因表达谱/RNA测序/信号转导Key words
weightlessness/mice/common carotid arteries/vascular remodeling/extracellular matrix/gene expression profiling/RNA-Seq/signal transduction分类
医药卫生引用本文复制引用
巩航滨,杨凡,王昱,李博文,陈琨,王莉..电磁悬吊间歇性失重与体液重分布耦合作用诱导小鼠颈总动脉重塑及干预靶点筛选[J].空军军医大学学报,2026,47(1):11-19,9.基金项目
国家自然科学基金青年科学基金(82302090) （82302090）
陕西省自然科学基础研究计划重点项目(2022JZ-53) （2022JZ-53）
