空军军医大学学报2026，Vol.47Issue(1)：20-26,7.DOI:10.13276/j.issn.2097-1656.2026.01.003
Cx43在模拟失重致血管平滑肌细胞表型转换中的作用
Effects of Cx43 on phenotypic switching of vascular smooth muscle cells under simulated microgravity
摘要
Abstract
Objective To investigate the effects of connexin 43(Cx43)on phenotypic switching of vascular smooth muscle cells under simulated microgravity.Methods Human arterial smooth muscle cells(HASMCs)were cultured under simulated microgravity by a cell stress-loading culture system and randomly divided into normal gravity group(NC group)and simulated microgravity group(SMG group).After 96 h of cyclic stretch,differential proteins were screened by mass spectrometry.Expression of α-smooth muscle actin(α-SMA)and smooth muscle protein 22-α(SM22α),osteopontin(OPN)and Cx43 in HASMCs was determined by Western blotting,and their migratory capacity was evaluated with Transwell assays.Following the construction of Cx43 knockdown cells using shRNA lentiviral vectors and a subsequent 96-hour cyclic stretch,their function was assessed.Results Proteomic profiling revealed that compared with the NC group,SMG group significantly down-regulated contractile proteins ACTA2 and TAGLN,while up-regulating migration-related proteins LPAR1,TGFBR1,PDGFD,and GJA1.Moreover,they were significantly enriched in positive regulators of cell migration and cytokine-cytokine interaction.Further experimental results showed that compared with the NC group,the levels of α-SMA and SM22α in HASMCs of the SMG group were markedly decreased(P<0.05),the expression of OPN was significantly increased(P<0.05),and the number of migrating cells increased significantly(P<0.05).Cx43 mRNA and protein levels in HASMCs of the SMG group were also significantly higher than those of the NC group(P<0.05).Knockdown of Cx43 significantly increased α-SMA and SM22α expression(P<0.05),and significantly reduced OPN levels(P<0.05)in the SMG group.The results of the Transwell aassay indicated that knockdown of Cx43 could inhibit the migration ability of HASMCs.Conclusion Simulated microgravity promotes smooth muscle cells phenotypic switching and migratory capacity via a Cx43-dependent pathway.关键词
模拟失重/Cx43/血管平滑肌细胞/蛋白组学/表型转换/迁移能力/机械应力/转染Key words
simulated microgravity/Cx43/vascular smooth muscle cells/proteomics/phenotypic switching/migration ability/mechanical stress/transfection分类
医药卫生引用本文复制引用
李曦,李娜,李程飞,张倩,赵子怡,王永春..Cx43在模拟失重致血管平滑肌细胞表型转换中的作用[J].空军军医大学学报,2026,47(1):20-26,7.基金项目
国家自然科学基金面上项目(3207090219) （3207090219）
