中国病理生理杂志2026，Vol.42Issue(1)：38-44,7.DOI:10.3969/j.issn.1000-4718.2026.01.005
基于嵌套阵列芯片的胃癌类器官模型构建及其在药敏检测中的应用研究
Construction and application of gastric cancer organoids using a nested array chip for drug sensitivity testing
摘要
Abstract
AIM:To construct a patient-derived gastric cancer organoid(PDO)model using a nested array chip and evaluate its utility in personalized drug screening.METHODS:Tissue specimens were obtained from eight gas-tric cancer patients during surgical resection.The specimens were digested,dissociated,and then mixed with 50%Matri-gel.An 8 µL aliquot of the mixture was seeded into the 3D implantation holes of the chip.Hematoxylin and eosin(HE)staining,along with immunohistochemical staining,were employed to validate the histomorphological and immunological marker consistency between the PDOs and the primary tumor tissues.Drug sensitivity screening was conducted using monotherapies of Paclitaxel,5-fluorouracil(5-FU),and oxaliplatin,as well as the 5-FU+oxaliplatin(FOLFOX)combina-tion regimen.The viability of the chip-based PDO model for drug screening was evaluated by integrating imaging analyses.RESULTS:Six PDO lines were successfully cultured,encompassing 2 intestinal-type,2 diffuse-type,and 2 mixed-type organoids.The PDOs displayed individual variations in morphology and growth characteristics.Notably,they exhibited identical morphological features and comparable immunological marker expression patterns to their corresponding primary tumors.More significantly,the drug response outcomes obtained from the chip-based model mirrored the heterogeneous re-sponses among individual patients,with the differential drug sensitivity profiles aligning well with the actual clinical re-sponses.CONCLUSION:The preliminary verification established the feasibility of utilizing PDOs on this nested array chip as an in vitro platform for drug screening.关键词
胃癌类器官/嵌套阵列芯片/个性化药物筛查/化疗Key words
gastric cancer organoids/nested array chip/personalized drug screening/chemotherapy分类
医药卫生引用本文复制引用
张灵玉,张麟腾,范红梅,李秋妹..基于嵌套阵列芯片的胃癌类器官模型构建及其在药敏检测中的应用研究[J].中国病理生理杂志,2026,42(1):38-44,7.基金项目
福建省自然科学基金资助项目(No.2022J05075) （No.2022J05075）
福建省卫生健康中青年骨干人才培养项目(No.2022GGA030) （No.2022GGA030）
福建创新联合基金项目(No.2021Y9198) （No.2021Y9198）
福建省肿瘤医院科研培育项目(No.2023YN08) （No.2023YN08）
