中草药2026，Vol.57Issue(3)：799-805,7.DOI:10.7501/j.issn.0253-2670.2026.03.002
杨树桑黄化学成分研究
Chemical constituents of Sanghuangporus vaninii
摘要
Abstract
Objective To investigate the chemical constituents from Sanghuangporus vaninii and their activity against tumor necrosis factor-α(TNF-α)-induced human rheumatoid arthritis fibroblast synovial cells(MH7A).Methods Isolation and purification were carried out by using various separation techniques such as silica gel,ODS column chromatography,and semi-preparative high-performance liquid chromatography.The structures of isolated compounds were elucidated through comprehensive spectroscopic analyses,such as UV,IR,HRESIMS,NMR,carbon spectrum calculation and ECD.Meanwhile,the activity of sesquiterpene compounds in inhibiting the proliferation of MH7A cells induced by TNF-α was evaluated by the CCK-8 method and the levels of inflammatory cytokines IL-6 and IL-1β were detected by ELISA.Results A total of nine compounds were isolated from 95%ethanol extract of S.vaninii and were identified as(4S,5S,6R)-2-methyl-5,6-dihydroxy-[R-6-(4-hydroxymethyl)-3-cyclohexene]-furan-2(5H)-one(1),phellilane D(2),phellilane C(3),(+)-γ-ionylideneacetic acid(4),elgonene A(5),(3E)-4-(3,4-dihydroxyphenyl)-but-3-en-2-one(6),3,4-dihydroxybenzoate(7),(E)-4-(4-hydroxyphenyl)but-3-en-2-one(8),protocatechualdehyde(9),respectively.The bioactivity results showed that compounds 1,3 and 4 significantly inhibited the proliferation of TNF-α-induced MH7A cells within 20 μmol/L,and the inhibition rates were(38.9±1.32)%、(46.21±0.86)%and(36.59±1.51)%at 20 μmol/L,respectively.Furthermore,the ELISA results showed they effectively reduced the production of IL-6 and IL-1β on TNF-α-induced MH7A cells.Conclusion Compounds 1-5 were identified as bisabolane type sesquiterpenoids and 6-9 were identified as phenols.Compound 1 was a new sesquiterpenoid and named as phellilane Q,Compound 7 was first isolated from S.vaninii.Compounds 1,3 and 4 significantly suppressed the proliferation of TNF-α-induced MH7A cells and demonstrated anti-inflammatory effects.关键词
桑黄/倍半萜/苯酚类化合物/抗类风湿关节炎/杨树桑黄萜素Q/phellilane C/(+)-γ-ionylideneacetic acidKey words
Sanghuangporus vaninii(Ljub)L.W.Zhou et Y.C.Dai/sesquiterpenoids/phenols/anti-rheumatoid arthritis/phellilane Q/phellilane C/(+)-γ-ionylideneacetic acid分类
医药卫生引用本文复制引用
任航洁,陶玉佳,李刚,李华强,秦路平,施琦..杨树桑黄化学成分研究[J].中草药,2026,57(3):799-805,7.基金项目
国家自然科学基金资助项目(U2202213) （U2202213）
国家自然科学基金资助项目(32470428) （32470428）
