中草药2026，Vol.57Issue(3)：806-816,11.DOI:10.7501/j.issn.0253-2670.2026.03.003
固公果果实干预胃炎-癌转化的活性成分研究
Active components of Rosa odorata var.gigantea fruits in intervening gastritis-cancer transformation
摘要
Abstract
Objective This study aimed to explore the bioactive constituents of Rosa odorata Sweet var.gigantea(Coll.et Hemsl.)Rehd.et Wils.fruits that intervene in the gastritis-cancer transformation process.Methods The transformation of human gastric mucosal epithelial cells(GES-1 cells)was induced by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG)to obtain human gastric mucosal epithelial dermatitis-cancer transformation model cells(MC cells).,The activity screening was conducted on the different polar fractions from the 60%ethanol extract of R.odorata var.gigantea fruits.Active fractions were isolated and purified using multiple chromatographic techniques,including silica gel column chromatography,ODS column chromatography,Sephadex LH-20 column chromatography,and preparative high-performance liquid chromatography(preparative HPLC).The chemical structures of the isolated compounds were elucidated based on physico-chemical properties and spectroscopic data.The CCK-8 assay was utilized to evaluate the anti-proliferative activity of the isolated monomeric compounds against MC cells.Results A total of 18 compounds were isolated and identified from the active fractions of R.odorata var.gigantea fruits,namely:(2R,3R)-3-[(1'S,2'R,6'S)-2',6'-dihydroxy-1'-(galloyl)cyclohexyl]-2,3-dihydroxypropanoic acid(1),microphyllose A(2),caffeic acid(3),3,5-dicaffeoylquinic acid(4),5-galloylquinic acid(5),ethyl vanillate(6),procyanidin B2(7),3-O-galloylquinic acid(8),gallocatechin gallate(9),6-O-galloyl-β-D-glucose(10),methyl gallate(11),3,4,5-tri-O-galloylquinic acid(12),ethyl gallate(13),3,5,4'-trihydroxystilbene(14),quercitrin(15),quercetin(16),luteolin(17),and betulinic acid(18).The results of the activity assay indicated that compounds 1,9,14-18 with an inhibition rate exceeding 50%exhibited the following IC50 values:93.47,81.79,37.44,81.58,86.01,44.02,and 66.12 μmol/L.Conclusion Compound 1 is a novel compound,designated as gugongguoic acid A.Compound 2 was isolated from the genus Rosa for the first time.Compounds 5,9,11,13,15,and 17 were isolated from this plant species for the first time.All 18 compounds exhibited inhibitory activity against MC cell proliferation.Among them,compounds 1,9,14-18 showed inhibition rates greater than 50%,which represent potential bioactive constituents for intervening in the gastritis-cancer transformation process.关键词
固公果/胃炎-癌转化/固公果酸A/5-没食子酰奎宁酸/没食子儿茶素没食子酸酯/槲皮苷/木犀草素Key words
Rosa odorata Sweet var.gigantea(Coll.et Hemsl.)Rehd.et Wils./gastritis-cancer transformation/gugongguoic acid A/5-galloylquinic acid/gallocatechin gallate/quercitrin/luteolin分类
医药卫生引用本文复制引用
陈宇航,姚彬,尹孝荣,原震,张静泽,刘岱琳..固公果果实干预胃炎-癌转化的活性成分研究[J].中草药,2026,57(3):806-816,11.基金项目
天津市科技计划项目(24ZXZSSS00260) （24ZXZSSS00260）
天津市科技计划项目(25ZYCGCG00390) （25ZYCGCG00390）
