中草药2026，Vol.57Issue(3)：817-824,8.DOI:10.7501/j.issn.0253-2670.2026.03.004
大蕉叶化学成分及细胞增殖抑制活性研究
Chemical constituents from leaves of Musa×paradisiaca and their anti-proliferative activities
摘要
Abstract
Objective To investigate the chemical constituents from the leaves of Musa×paradisiaca and their inhibitory activities on cell proliferation in vitro.Methods The compounds were isolated and purified using various chromatographic techniques,including silica gel,MCI gel CHP-20P,reversed-phase ODS,Sephadex LH-20 column chromatographies,and semi-preparative HPLC.Their structures were elucidated by spectroscopic methods such as NMR and MS.The inhibitory activities of all compounds on cell proliferation in vitro were evaluated using the CCK-8 assay.Results A total of 19 compounds were isolated from the leaves of Musa×paradisiaca and identified as di(2-ethylhexyl)phthalate(1),myristic acid(2),pentadecanoic acid(3),linoleic acid(4),dibutyl phthalate(5),ethyl 4-hydroxy-3-methoxybenzoate(6),dihydroactinidolide(7),MF-EA-705β(8),butyl 2-ethylhexyl phthalate(9),n-tetratriacont-20,23-dienoic acid(10),3-hydroxystigmasta-5,22-dien-7-one(11),3-hydroxystigmast-5-en-7-one(12),luffarin X(13),6-hydroxystigmasta-4,22-dien-3-one(14),6-hydroxystigmast-4-en-3-one(15),(3S,5R,6S,7E)-5,6-epoxy-3-hydroxy-7-megastigmen-9-one(16),ethyl p-hydroxybenzoate(17),p-diethoxybenzene(18),and methyl(9Z,12Z,15Z)-octadeca-9,12,15-trienoate(19).Among them,compounds 2,5,7,11,13,15,and 17 exhibited certain anti-proliferative activities against human hepatocellular carcinoma HepG2,cervical cancer Hela,breast cancer MCF-7,and malignant melanoma A375 cell lines,with median inhibition concentration(IC50)values ranging from 40.26 to 97.75 μmol/L.Specifically,compounds 7 and 11 showed selective inhibitory activity against A375 cells(IC50=43.0 and 50.0 μmol/L,respectively),which was slightly weaker than that of the positive control cisplatin(IC50=40.13 μmol/L).In contrast,compounds 2 and 15 demonstrated stronger inhibitory effects against MCF-7 cells(IC50=40.26 and 45.00 μmol/L,respectively)compared to cisplatin(IC50=48.36 μmol/L).Conclusion Compounds 1 and 6-19 were isolated from this genus for the first time,while compounds 2-5 were first identified from this specific plant.Furthermore,compounds 2,7,11,and 15 exhibited selective inhibitory activities against specific tumor cell lines.关键词
芭蕉属/大蕉叶/细胞增值抑制活性/邻苯二甲酸二(2-乙基)己酯/二氢猕猴桃内酯/3-羟基豆甾-5,22-二烯-7-酮/6-羟基豆甾-4-烯-3-酮/(9Z,12Z,15Z)-十八烷基-9,12,15-三烯酸甲酯Key words
Musa L./leaves of Musa×paradisiaca L./anti-proliferative activity/di(2-ethylhexyl)phthalate/dihydroactinidolide/3-hydroxystigmasta-5,22-dien-7-one/6-hydroxystigmast-4-en-3-one/methyl(9Z,12Z,15Z)-octadeca-9,12,15-trienoate分类
医药卫生引用本文复制引用
严绪华,孙佳乐,梁秋明,王峰,方振峰..大蕉叶化学成分及细胞增殖抑制活性研究[J].中草药,2026,57(3):817-824,8.基金项目
广东省普通高校重点领域专项(2022ZDZX2035) （2022ZDZX2035）
