中草药2026，Vol.57Issue(3)：859-870,12.DOI:10.7501/j.issn.0253-2670.2026.03.007
基于中药超分子探讨黄芪-莪术配伍增效抗肝癌的科学内涵
A discussion on scientific connotation of synergistic effect of Astragali Radix and Curcumae Rhizoma in enhancing anti-liver cancer effect based on traditional Chinese medicine supramolecular
摘要
Abstract
Objective Based on the supramolecular of traditional Chinese medicine(STCM)theory,this study systematically explored the material basis of Huangqi(Astragali Radix)-Ezhu(Curcumae Rhizoma)(AC)herb pair for synergistic anti-liver cancer,aiming to reveal the scientific connotation of its compatibility.Methods The STCM were separated by the centrifugation-dialysis method.The particle size and morphology were characterized by dynamic light scattering(DLS)technology and transmission electron microscopy(TEM),and the formation mechanism was analyzed by spectroscopic technology.The chemical composition was further analyzed by liquid chromatography-mass spectrometry(LC-MS).Its anti-liver cancer activity in vitro was evaluated.Results The results showed that STCM existed in both the AC co-decoction and the physically mixed solution,but the STCM in the co-decoction(AC-STCM)had smaller particle size,more uniform distribution,and higher stability;its self-assembly process may be jointly driven by components such as calycosin,formononetin,and demethoxycurcumin through hydrogen bonding and π-π stacking interactions.AC-STCM exhibited significant inhibitory effects on HepG2 cells(P<0.01),with its in vitro antitumor activity notably superior to that of the co-decoction and the physically mixed STCM(Mix-STCM)(P<0.01),while the co-decoction itself also showed better antitumor effects than the physical mixture(P<0.01).Conclusion During the decoction process,AC herb pairs self-assemble through non-covalent bonds to form a stable STCM system,which constitutes an important material basis for their synergistic anti-liver cancer effects.This study is the first to explain the scientific connotation of AC for synergistic anti-liver cancer from the perspective of STCM,which provides a theoretical basis and experimental basis for the modernization of the theory of compound compatibility of traditional Chinese medicine.关键词
中药超分子/黄芪/莪术/药对/配伍/物质基础/LC-MS/毛蕊异黄酮/芒柄花素/双去甲氧基姜黄素/抗肿瘤Key words
supramolecules of traditional Chinese medicine/Astragali Radix/Curcumae Rhizoma/herb pairs/compatibility/material basis/LC-MS/calycosin/formononetin/demethoxycurcumin/antitumor activity分类
医药卫生引用本文复制引用
刘若雨,王祖驰,刘名玉,赵春芹,张振,李肖..基于中药超分子探讨黄芪-莪术配伍增效抗肝癌的科学内涵[J].中草药,2026,57(3):859-870,12.基金项目
国家自然科学基金青年项目(82204656) （82204656）
博士后科学基金特别资助(2024T170534) （2024T170534）
山东省中医药科技项目(M20241725) （M20241725）
山东中医药大学青年创新团队支持计划(22202105) （22202105）
