中草药2026，Vol.57Issue(3)：885-896,12.DOI:10.7501/j.issn.0253-2670.2026.03.009
基于层次分析-熵权法的经典名方白术散提取工艺优化及其抗炎活性验证
Extraction process optimization and anti-inflammatory activity validation of classic prescription Baizhu Powder using analytic hierarchy process-entropy weight method
摘要
Abstract
Objective To optimize the extraction process of the classic prescription Baizhu Powder(白术散),and verify its anti-inflammatory activity through cell anti-inflammatory activity experiments,providing a basis for the development of Baizhu Powder.Methods An HPLC detection method was established for the index components puerarin,daidzin,ginsenoside Rg1,daidzein,ginsenoside Rb1,and ammonium glycyrrhizinate.Taking the content of index components,paste yield and oil extraction volume as evaluation indicators,the comprehensive weights of each index were determined by analytic hierarchy process(AHP)-entropy weight method.Based on the single-factor experiment,the Box-Behnken design-response surface method(BBD-RSM)was adopted to optimize the Baizhu Powder extraction process.The anti-inflammatory activity of Baizhu Powder was investigated by inducing an inflammatory model of human normal colonic epithelial cells(NCM460)with lipopolysaccharide(LPS),and the feasibility of the process was verified.Results The optimized extraction process of Baizhu Powder was 10 times the volume of water,soaking for 30 min,extraction time for 90 min,and extraction twice.Under these conditions,the average comprehensive score of the three batches of process validation was 0.974,and the RSD value was 1.94%.The pharmacodynamic verification results of Baizhu Powder indicated that the Baizhu Powder prepared by the optimized process could significantly reduce the levels of inflammatory factors interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in the LPS-induced NCM460 cell inflammatory model.The level of the anti-inflammatory factor IL-10 was increased,and there was no significant difference compared with the Baizhu Powder benchmark sample.Conclusion The Baizhu Powder extraction process optimized by AHP-entropy weight method combined with BBD-RSM is stable and reliable,which can provide a basis for the subsequent research and development of Baizhu Powder.关键词
白术散/Box-Behnken设计-响应面法/AHP-熵权法/葛根素/大豆苷/人参皂苷Rg1/大豆苷元/人参皂苷Rb1/白细胞介素/肿瘤坏死因子-αKey words
Baizhu Powder/Box-Behnken design-response surface method/AHP-entropy weight method/puerarin/daidzin/ginsenoside Rg1/daidzein/ginsenoside Rb1/interleukin/tumor necrosis factor-α分类
医药卫生引用本文复制引用
郝凌烽,刘义豪,王婷婷,王丹凤,胡晓红,臧振中,吴样明,朱卫丰,管咏梅..基于层次分析-熵权法的经典名方白术散提取工艺优化及其抗炎活性验证[J].中草药,2026,57(3):885-896,12.基金项目
全国重点实验室专项(2023QGZDSYS002) （2023QGZDSYS002）
2024江西省重点研发项目(20243BCC31010) （20243BCC31010）
经典名方研发与国际拓展研究(20232BBH80009) （20232BBH80009）
