中草药2026，Vol.57Issue(3)：897-910,14.DOI:10.7501/j.issn.0253-2670.2026.03.010
何首乌"九蒸九晒"炮制过程"颜色-成分-抗氧化活性"的变化及相关性研究
Changes and correlations of"color-component-antioxidant activity"in"nine-steaming and nine-sun-drying"processing of Polygoni Multiflori Radix
摘要
Abstract
Objective To investigate the correlation between intrinsic components,visual color,and antioxidant activity during the"nine-steaming and nine-sun-drying"processing of Heshouwu(Polygoni Multiflori Radix,PMR),providing a reference for determining the processing degree and quality control.Methods A colorimeter was used to objectively quantify the color of samples at different steaming and sun-drying cycles.HPLC was used to establish fingerprints,HPLC-Q-TOF-MS was employed to analysis characteristic peaks.Alcohol-soluble extract content was determined according to the Chinese Pharmacopoeia(2025 edition).Polysaccharide content was measured using the phenol-sulfuric acid method.Antioxidant activity was evaluated through DPPH radical scavenging capacity,ABTS radical scavenging assays,and ferric reducing antioxidant power(FRAP)method to determine total antioxidant capacity.Pearson correlation and regression analyses were conducted between the components,color values,and antioxidant activity.Cluster analysis,principal component analysis(PCA),and partial least squares regression(PLSR)were applied to determine the processing stages of"nine-steaming and nine-sun-drying"PMR,while entropy-weighted TOPSIS was used to identify its the optimal processing endpoint.Results During processing,the sample color gradually darkened,and chromaticity values decreased.Polysaccharide content increased progressively,while alcohol-soluble extract content first increased and then declined.A total of 26 chromatographic peaks were identified in the fingerprints,and the components of 25 of them were identified.Correlation analysis revealed significant relationships between intrinsic components,color values,and antioxidant activity.The processing stages were divided into initial,middle,and final phases,with the seven steaming and sun-drying cycle achieving the highest comprehensive score.Conclusion Significant changes in component content,color,and antioxidant activity occur during the processing of PMR,with notable correlations among them.The integrated"color-component-antioxidant activity"analysis enables objective discrimination of processing techniques and quality control in"nine-steaming and nine-sun-drying"PMR.关键词
何首乌/九蒸九晒/颜色-成分-抗氧化活性/相关性分析/炮制/没食子酸/5-HMF/二苯乙烯苷/大黄素-8-O-β-D-葡萄糖苷/大黄素甲醚-8-O-β-D-葡萄糖苷/大黄素/大黄素甲醚Key words
Polygoni Multiflori Radix/nine-steaming and nine-sun-drying/color-component-antioxidant activity/correlation analysis/processing/gallic acid/5-HMF/stilbenes glycosides/emodin-8-O-β-D-glucoside/physcion-8-O-β-D-glucoside/emodin/physcion分类
医药卫生引用本文复制引用
唐林,高元航,李海英,廖卓,余亚胜,王璐,杨磊,胡超..何首乌"九蒸九晒"炮制过程"颜色-成分-抗氧化活性"的变化及相关性研究[J].中草药,2026,57(3):897-910,14.基金项目
湖南省自然科学基金资助项目(2024JJ8151) （2024JJ8151）
湖南省自然科学基金资助项目(2024JJ6362) （2024JJ6362）
湖南省自然科学基金资助项目(2025JJ60642) （2025JJ60642）
中药创新能力提升项目(湘中医药函[2025]5号) （湘中医药函[2025]5号）
湖南省卫生健康高层次人才项目(湘卫函[2024]43、182号) （湘卫函[2024]43、182号）
国家中医药管理局全国老药工传承工作室建设项目(国中医药人教函[2024]255号) （国中医药人教函[2024]255号）
