针刺研究2026，Vol.51Issue(1)：31-38,8.DOI:10.13702/j.1000-0607.20241228
基于Ang-1/Tie-2通路探讨朱琏抑制Ⅱ型针法对缺血性中风大鼠脑血管新生的影响
Effects of Zhu Lian's type Ⅱ inhibition needling on cerebral angiogenesis in rats with ischemic stroke based on the Ang-1/Tie-2 pathway
摘要
Abstract
Objective To observe the effects of Zhu Lian's type Ⅱ inhibition needling on neurological function and angiogenesis in rats with ischemic stroke(IS),so as to explore its potential mechanisms.Methods Wistar rats were divided into sham operation group,model group,acupuncture group,and acupuncture+angiogenin(Ang)-2 group,with 12 rats in each group.A suture method was used to establish the IS model.The acupuncture group received Zhu Lian's type Ⅱ inhibition needling at the"Guanyuan"(CV4),"Guilai"(ST29),"Zusanli"(ST36),"Sanyinjiao"(SP6),and"Fenglong"(ST40)acupoints for 30 min per session.Rats in the acupuncture+Ang-2 group were injected via the tail vein with Ang-2 recombinant protein(10 mg/kg)before Zhu Lian's type Ⅱ inhibition needling intervention.All treatments were administered once daily for 7 consecutive days.Neurological deficit scores were assessed in rats of each group.TTC staining was employed to determine the percentage of cerebral infarction volume.HE staining was used to observe the pathological changes in the ischemic penumbra cortex.Immunohistochemistry was used to evaluate microvascular density in the ischemic penumbra cortex.Western blot was conducted to detect the expression levels of vascular endothelial growth factor(VEGF),vascular endothelial growth factor receptor 2(VEGFR-2),Ang-1,Ang-2,tyrosine kinase 2(Tie-2),and phosphorylated Tie-2(p-Tie-2)proteins in the ischemic penumbra cortex.Results Compared with the sham operation group,the model group showed elevated neurological deficit scores,cerebral infarction volume percentage,microvascular density in the ischemic penumbra cortex,and protein levels of VEGF,VEGFR-2,Ang-1,and p-Tie-2/Tie-2 ratio.Conversely,Ang-2 protein level was decreased(P<0.05).Compared with the model group and the acupuncture+Ang-2 group,rats in the acupuncture group had lower neurological deficit scores,cerebral infarction volume percentage,and Ang-2 protein level.While microvascular density in the ischemic penumbra cortex and the protein levels of VEGF,VEGFR-2,Ang-1,and the p-Tie-2/Tie-2 ratio were further increased(P<0.05).In the model group,the number of neurons in the ischemic penumbra cortex of rats was decreased,the nuclei were shrank or disappeared,the cell bodies were vacuolated and degenerated,accompanied by a large number of inflammatory cell infiltrations.Compared with the model group,the injury of cortical neurons in the acupuncture group was alleviated.Compared with the acupuncture group,the injury of cortical neurons in the acupuncture+Ang-2 group was aggravated.Conclusion Zhu Lian's type Ⅱ inhibition needling can improve neurological function in IS rats,possibly by activating the Ang-1/Tie-2 pathway to promote angiogenesis.关键词
缺血性中风/朱琏抑制Ⅱ型针法/血管新生/血管生成素-1/酪氨酸激酶2信号通路Key words
Ischemic stroke/Zhu Lian's type Ⅱ inhibition needling/Angiogenesis/Angiopoietin-1/tyrosine kinase 2 signaling pathway引用本文复制引用
翟阳,黎芳,莫智珍,梅小平,韦玉鲁,池腾飞,邹敏,庞延,王瀚..基于Ang-1/Tie-2通路探讨朱琏抑制Ⅱ型针法对缺血性中风大鼠脑血管新生的影响[J].针刺研究,2026,51(1):31-38,8.基金项目
广西壮族自治区中医药管理局自筹课题项目(No.GXZYA20230437) （No.GXZYA20230437）
中国博士后科学基金资助项目(No.2024MD753922) （No.2024MD753922）
广西自然科学基金项目(No.2024GXNSFAA010252) （No.2024GXNSFAA010252）
广西青年岐黄学者培养项目(No.GXQH202419) （No.GXQH202419）
