中国人兽共患病学报2025，Vol.41Issue(12)：1262-1268,7.DOI:10.3969/j.issn.1002-2694.2025.00.176
牛分枝杆菌减毒株卡介苗感染巨噬细胞触发GSDMD介导细胞焦亡的研究
Mycobacterium bovis attenuated strain BCG induces GSDMD-mediated pyroptosis in mouse macrophages
摘要
Abstract
This study elucidated the role of GSDMD mediated pyroptosis in attenuated Mycobacterium bovis strain BCG infection.Macrophages were infected with BCG(MOI=10),and cells were collected at various time points for detection of protein levels of NLRP3,cleaved caspase-1,and GSDMD-N.The formation of ASC specks was also detected.qPCR and ELISA were used to deter-mine IL-1β mRNA and protein levels after BCG infection.Finally,the percentage cell death in BCG-infected bone marrow-derived macrophages from GSDMD knockout mice was detected with pyridine iodide staining.The attenuated M.bovis strain BCG induced a significant increase in NLRP3 protein levels after infecting macrophages.NLRP3 formed a platform by binding ASC,and subsequently recruited pro-caspase-1 and activated caspase-1.Consequently,BCG activated GSDMD and increased IL-1β secretion,thus ulti-mately inducing pyroptosis,a critical process in BCG clearance.This study demonstrated that BCG infection induces GSDMD-mediated pyroptosis in mouse macrophages,and this pyroptosis restricts BCG survival.The results provide a theoretical basis for fur-ther understanding of the mechanism of BCG-induced protective immunity and modification of the BCG vaccine.关键词
牛分枝杆菌/BCG/巨噬细胞/细胞焦亡/GSDMDKey words
Mycobacterium bovis/BCG/macrophage/pyroptosis/GSDMD分类
医药卫生引用本文复制引用
唐丽,宋银娟,李娜,马慧,张正洋,岳彩凤,刘思岐,张在刚,储岳峰..牛分枝杆菌减毒株卡介苗感染巨噬细胞触发GSDMD介导细胞焦亡的研究[J].中国人兽共患病学报,2025,41(12):1262-1268,7.基金项目
中央高校基本科研业务费专项资金项目(No.lzujbky-2024-it63) （No.lzujbky-2024-it63）
国家重点研发计划项目(No.2023YFD1800703) （No.2023YFD1800703）
甘肃省自然科学基金(No.25JRRA438) （No.25JRRA438）
甘肃省科技重大专项(No.22ZD6NA001) （No.22ZD6NA001）
中国农业科学院创新工程项目(No.CAAS-ASTIP-2021-LVRI) Fundamental Research Funds for the Central Universities(No.lzujbky-2024-it63) （No.CAAS-ASTIP-2021-LVRI）
National Key Research and De-velopment Program of China(No.2023YFD1800703) （No.2023YFD1800703）
Natural Science Foundation of Gansu Province(No.25JRRA438) （No.25JRRA438）
Major Sci-ence and Technology Program of Gansu Province(No.22ZD6NA001) （No.22ZD6NA001）
Innovation Program of Chinese Academy of Agricultural Sci-ences(No.CAAS-ASTIP-2021-LVRI) （No.CAAS-ASTIP-2021-LVRI）
