中国人兽共患病学报2025，Vol.41Issue(12)：1283-1288,6.DOI:10.3969/j.issn.1002-2694.2025.00.179
福建地区猪肉源fosA7基因阳性沙门菌流行及耐药特征分析
Epidemiological and antimicrobial resistance characteristics analysis of fosA7-positive Salmonella from pork in Fujian Province
摘要
Abstract
This study investigated the epidemiological characteristics of the fosA7 gene in Salmonella isolated from pork in Fujian Province,to provide a theoretical foundation for controlling the dissemination of Salmonella antibiotic resistance.This study isolated 121 strains of Salmonella from pork in Fujian.Whole-genome sequencing technology was used to detect antimicrobial resistance genes,plas-mid types,serotypes,and multilocus sequence types(MLST).Additionally,the agar dilution method was used for antibiotic susceptibil-ity testing.A total of 32 strains(26.45％of the total isolates)were found to carry the fosfomycin resistance gene fosA7.Among the fosA7-positive Salmonella strains,27 antimicrobial resistance genes,2 serotypes,2 sequence types(STs),and 8 plasmid types were identified.The carriage rates of resistance genes were highest for the quinolone resistance gene qnrS1 and the phenicol resistance gene floR(78.13％,25/32).Salmonella Derby(93.75％,30/32)emerged as the dominant serotype,whereas ST40(93.75％,30/32)was the prevalent ST.Col-RNAI was the most prevalent plasmid(40.63％,13/32).Antibiotic susceptibility testing indicated that fosA7-positive Salmonella strains exhibited high resistance rates(exceeding 50％)to florfenicol,ampicillin,amoxicillin/clavulanic acid,and doxycycline.These findings indicate widespread prevalence of the fosA7 gene in Salmonella isolated from pork in Fujian Province.The MIC values of fosA7-positive strains against fosfomycin were higher than those of fosA7-negative strains.Additionally,high carriage rates of genes conferring resistance to quinolones and sulfonamides were observed,coupled with severe resistance to antimicrobials such as florfenicol and ampicillin.These findings suggest a need for enhanced monitoring and prevention and control measures for Salmonella.关键词
沙门菌/fosA7基因/磷霉素/耐药基因Key words
Salmonella/fosA7 gene/fosfomycin/antimicrobial resistance gene分类
医药卫生引用本文复制引用
叶欣颖,杨守深,陈海贞,李晓会,罗翠雅,陈子涛,陈巧芸,邓秋铭,李兰,范克伟..福建地区猪肉源fosA7基因阳性沙门菌流行及耐药特征分析[J].中国人兽共患病学报,2025,41(12):1283-1288,6.基金项目
福建省自然科学基金(No.2022J011156) （No.2022J011156）
龙岩市科技计划项目(No.2022LYF9001) （No.2022LYF9001）
漳平市奇迈科技创新基金项目(漳工科No.[2021]123号) （漳工科No.[2021]123号）
龙岩市新罗区产学研科技联合创新项目(No.2022XLXYZ009) Natural Science Foundation Project of Fujian Province(No.2022J011156) （No.2022XLXYZ009）
Longyan Science and Technology Project(No.2022LYF9001) （No.2022LYF9001）
Zhangping Qimai Science and Technology Innovation Fund Project(No.[2021]123) （No.[2021]123）
Industry-University-Research & Science and Technology Project of Xinluo District,Longyan(No.2022XLXYZ009) （No.2022XLXYZ009）
