中国人兽共患病学报2025，Vol.41Issue(12)：1295-1299,5.DOI:10.3969/j.issn.1002-2694.2025.00.175
改良抗酸菌荧光染色镜检试剂的研究
Modification of reagents for fluorescence staining microscopic examination of acid-fast bacilli
摘要
Abstract
To decrease the use of hazardous chemicals,this study reports an evaluation of the staining effects of using Triton X-100-Vaseline instead of phenol,using methylene blue instead of potassium permanganate,and reduced amounts of alcohol and hydro-chloric acid to prepare a decolorization solution for acid fast bacterial fluorescence staining.Sputum samples were collected from pa-tients with suspected pulmonary tuberculosis.Smear staining microscopic examination and MGIT960 fluid culture were performed ac-cording to standard tuberculosis laboratory testing procedures.A Triton X-100-Vaseline water suspension was used instead of a phe-nol water suspension to prepare the initial staining solution(auramine O working solution),potassium permanganate counterstaining solution was replaced with 0.1％methylene blue,and 5％hydrochloric acid(95％alcohol)was replaced with 0.5％hydrochloric acid(75％alcohol)as the decolorization solution.After staining and microscopic examination,the sensitivity and specificity of the new re-agents were compared with the standard staining method according to culture results.The standard staining results had a sensitivity and specificity of 57.14％(40/70)and 98.69％(151/153),respectively.The sensitivity and specificity of Triton X-100-Vaseline were 55.71％(39/70)and 98.04％(150/153),respectively.The sensitivity and specificity of the 0.5％hydrochloric acid 75％alcohol method were 57.14％(40/70)and 98.04％(150/153),respectively.The sensitivity and specificity of the 0.1％methylene blue method were 55.71％(39/70)and 98.04％(150/153),respectively,No significant differences in the results were observed for the three modi-fied reagents versus the standard method(P＞0.05).On the basis of the evaluation data,in acid fast fluorescence staining,Triton X-100-Vaseline can replace phenol in preparation of the initial staining solution.In the decolorization solution,0.5％hydrochloric acid-75％alcohol can be used,and 0.1％methylene blue aqueous solution can be used as a potassium permanganate substitute for counter-staining.关键词
抗酸菌/染色/危险化学品Key words
acid fast bacilli/staining/hazardous chemicals分类
医药卫生引用本文复制引用
姜广路,黄海荣..改良抗酸菌荧光染色镜检试剂的研究[J].中国人兽共患病学报,2025,41(12):1295-1299,5.基金项目
北京市卫生健康委员会高层次公共卫生人才(学科带头人)(No.G2023-2-002) Beijing Municipal Health Commission High-level Public Health Talent(Academic leader)(No.G2023-2-002) （学科带头人）
