中国临床药理学杂志2025，Vol.41Issue(21)：3043-3048,6.DOI:10.13699/j.cnki.1001-6821.2025.21.008
败酱总黄酮调节RhoA/ROCK通路对缺血性脑卒中大鼠神经保护的研究
Research on the neuroprotective effect of total flavonoids of Patrinia scabra on rats with ischemic stroke by regulating the RhoA/ROCK pathway
摘要
Abstract
Objective To investigate the effect of total flavonoids from Patrinia on neuroprotection in rats with ischemic stroke(IS)by Rat sarcoma virus oncogene homolog(Ras)homolog gene family member(Rho)A/Rho-associated kinase(ROCK)signaling pathway.Methods The IS rat model was established by the suture method,and was randomly divided into B group(IS),C group(total senna flavonoids low dose),D group(total senna flavonoids high dose),E group(positive drug),F group(high dose of total senna flavonoids+U-46619F)and A group(the sham operation),where group A and group B were given intragastric and intraperitoneal injections of normal saline;group C was given 100 mg·kg-1 total senna flavonoids by intragastric administration;group D was given 200 mg·kg-1total senna flavonoids by intragastric administration;group E was given 1.0 mg·kg-1 nimodipine by intraperitoneal injection;group F was given intragastric administration of 200 mg·kg-1 total senna flavonoids and 30 μg·kg-1 U-46619 by intraperitoneal injection;each group was administered once a day for two consecutive weeks.After the intervention,the modified neurological severity(mNSS)score was used to evaluate the nerve injury;the triphenyltetrazolium chloride staining(TTC)was used to detect the ratio of cerebral infarction area;the brain tissue was separated,and hematoxylin-eosin was used for pathological changes;the expression of nerve growth factor(NGF)was detected by immunohistochemistry;the apoptosis changes of striatal dopaminergic neurons were detected by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling method(TUNEL);the relative expression levels of Bcl-2 related X protein(Bax),RhoA,ROCK and B-cell lymphoma factor 2(Bcl-2)proteins were detected by immunoblotting.Results The nerve injury scores for groups A,B,D,E,and F were(0.15±0),(11.24±1.26),(4.06±0.42),(3.52±0.38)and(7.18±0.73)points,respectively;the ratio of infarct area were(0±0)％,(27.86±2.81)％,(11.57±1.27)％,(12.07±1.24)％and(18.07±1.88)％;the relative expression levels of NGF were 34.11±3.51,15.86±1.61,32.07±3.28,32.11±3.32 and 22.87±2.31;the apoptosis of dopaminergic neurons were(6.57±0.68)％,(27.81±2.82)％,(12.75±1.31)％,(12.52±1.27)％and(20.47±2.11)％;the aforementioned indicators of group B were compared with those of group A;those of groups C and D were compared with group B;and those of group F were compared with group D,all showing statistically significant differences(all P＜0.05).Conclusion The total flavonoids of Cynanchum paniculatum have neuroprotective effects on IS rats,and the mechanism may be related to the activation of the RhoA/ROCK signaling pathway.关键词
败酱总黄酮/缺血性脑卒中/Ras同源基因家族成员A/Rho相关激酶信号通路/神经保护Key words
total flavonoids of Patrinia scabra/ischemic stroke/Ras homolog gene family member A/Rho-associated kinase signaling pathway/neuroprotection分类
医药卫生引用本文复制引用
聂群成,贾晓彤,刘鹏飞..败酱总黄酮调节RhoA/ROCK通路对缺血性脑卒中大鼠神经保护的研究[J].中国临床药理学杂志,2025,41(21):3043-3048,6.基金项目
济源市科技发展计划基金资助项目(20023004) （20023004）
