中国兽医科学2026，Vol.56Issue(1)：13-19,7.DOI:10.16656/j.issn.1673-4696.2026.0004
口蹄疫病毒3D蛋白与宿主互作蛋白的筛选及鉴定
Screening and identification of host proteins interacting with 3D protein of foot-and-mouth disease virus
摘要
Abstract
Foot-and-mouth disease(FMD)is an acute,febrile,and highly contagious infectious dis-ease of cloven-hoofed animals such as pigs,cattle,and sheep,caused by foot-and-mouth disease virus(FMDV).The FMDV 3D protein is the RNA-dependent RNA polymerase(3Dpol)encoded by the virus,which is crucial for viral RNA replication.However,there are few reports on the interaction between 3D and host proteins.In this study,a yeast two-hybrid system was used with a cDNA yeast expression library from porcine kidney epithelial PK-15 cells,and 15 potential host proteins interacting with 3D were ulti-mately obtained.Further validation by co-immunoprecipitation and confocal microscopy identified that host ataxin-3 and VDAC1 interact with 3D.The findings provide data support for the role of host protein-3D interactions in viral replication.关键词
口蹄疫病毒/3D蛋白/酵母双杂交技术/蛋白质相互作用Key words
foot-and-mouth disease virus/3D protein/yeast two-hybrid technique/protein-protein interaction分类
农业科技引用本文复制引用
刘永杰,兰喜,李玉星,张勇,何继军,尚佑军,郑海学..口蹄疫病毒3D蛋白与宿主互作蛋白的筛选及鉴定[J].中国兽医科学,2026,56(1):13-19,7.基金项目
国家重点研发计划项目(2021YFD1800300) （2021YFD1800300）
国家生猪技术创新中心先导科技项目(NCTIP-XD/C03) （NCTIP-XD/C03）
甘肃省科技特派员专项(24CXNA055) （24CXNA055）
