中国蔬菜Issue(1):40-48,9.DOI:10.19928/j.cnki.1000-6346.2025.2056
花粉磁转染介导的植物遗传转化技术及其在蔬菜中的应用
Plant Transformation Technique Mediated by Pollen Magnetofection and Its Application in Vegetables
摘要
Abstract
Transgenetic technologies are crucial for the improvement of crop agronomic traits,yield,and quality.However,conventional transgenic methods face multiple limitations in biological breeding of vegetable crops.Through the method of pollen magnetofection,the exogenous nucleic acids can be directly delivered into pollen grains,and the transformation can be realized by pollination-fertilization.Due to its easy operation and tissue culture-free,pollen magnetofection is thought as one of new transformation techniques with promising application prospects.In this review,the technical principles,application advantages,practical implementation,and current challenges of pollen magnetofection in the transform of vegetable crops,are systematically introduced,and its future improvements are suggested,in order to provide a reference for efficient biological breeding in vegetable species.关键词
磁转染/花粉/遗传转化/蔬菜/生物育种Key words
magnetofection/pollen/genetic transformation/vegetable/biological breeding引用本文复制引用
范海玮,雍康,王振泽,逯明辉..花粉磁转染介导的植物遗传转化技术及其在蔬菜中的应用[J].中国蔬菜,2026,(1):40-48,9.基金项目
国家大宗蔬菜产业技术体系专项(CARS-23-G22),陕西省重点研发计划项目(2025NC-YBXM-043),陕西省农业核心技术攻关项目(2024NYGG008-2) (CARS-23-G22)