中国兽医科学2026，Vol.56Issue(1)：71-76,6.DOI:10.16656/j.issn.1673-4696.2026.0006
"卡盒式"非洲猪瘟病毒核酸一体化快检试剂的研制及应用
Development and application of an integrated"Cartridge-based"rapid nucleic acid detection kit for African swine fever virus
摘要
Abstract
One"cartridge-based"rapid nucleic acid detection kit for African swine fever virus(ASFV)is described in this paper,the kit integrates nucleic acid extraction reagents and fluorescent PCR reaction solutions into a fully enclosed"cartridge-based"reaction tube,enabling automated com-pletion of nucleic acid extraction and PCR amplification within 25 minutes.Performance evaluation demonstrated 100％specificity and a sensitivity of up to 608.5 copies/mL.The intra-and inter-assay coefficients of variation(CV)for medium and low concentration nucleic acid samples were both below 5％,indicating excellent repeatability and reproducibility.Validation with 90 known samples showed that,compared to the WOAH-recommended fluorescent PCR method(1.3.4 protocol),the kit achieved the concordance rates of 100％(41/41)for positive samples and 97.96％(48/49)for negative samples,with one negative sample tested to be positive with the cartridge-based kit,resulting in an overall concor-dance rate of 98.89％(89/90).Among 87 clinical samples of unknown infection status randomly collected from the market,the cartridge-based kit screened 14 positive samples,all of which were negative by the WOAH-recommended PCR.These findings suggest that the"cartridge-based"kit offers a superior tool for on-site rapid detection of ASFV with higher sensitivity and easily used.关键词
非洲猪瘟/现场快检/"卡盒式"一体化试剂Key words
African swine fever/on-site rapid detection/"cartridge-based"integrated kit分类
农业科技引用本文复制引用
史喜菊,杜思乐,高志强,张伟,赵相鹏,白子龙,赖平安,韦海涛,臧越鹏,汪琳,王素秋.."卡盒式"非洲猪瘟病毒核酸一体化快检试剂的研制及应用[J].中国兽医科学,2026,56(1):71-76,6.基金项目
"十四五"国家重点研发计划项目(2022YFD1800502) （2022YFD1800502）
