中国兽医科学2026，Vol.56Issue(1)：77-82,6.DOI:10.16656/j.issn.1673-4696.2025.0229
基于边缘无浆体msp4基因的PCR检测方法的建立及应用
Establishment and application of PCR detection method based on Anaplasma marginale msp4 gene
摘要
Abstract
To establish a specific,sensitive and rapid PCR assay for Anaplasma marginale,a primer pair targeting the conserved region of the msp4 gene was designed and the reaction conditions were opti-mized.The assay was then evaluated for sensitivity,specificity and repeatability,and applied to clini-cal samples.The method exclusively detected A.marginale DNA and showed no cross-reactivity with seven common blood-borne protozoa of domestic animals.The detection limit was 380 fg/μL(≈126 copies per re-action).Both intra-assay and inter-assay results indicated the method had high repeatability and sta-bility.Testing of 120 clinical samples revealed a positivity rate of 54.1％(65/120),significantly higher than the 37.5％(45/120)obtained with a previously reported PCR.In conclusion,the newly devel-oped PCR provides a sensitive,specific and rapid tool for the epidemiological surveillance and accurate diagnosis of bovine anaplasmosis in China.关键词
边缘无浆体/msp4基因/PCR/牛无浆体病Key words
Anaplasma marginale/msp4 gene/PCR/bovine anaplasmosis分类
农业科技引用本文复制引用
廖培淇,朱子奇,苏中华,甘富斌,李俊强,陈远才,张龙现..基于边缘无浆体msp4基因的PCR检测方法的建立及应用[J].中国兽医科学,2026,56(1):77-82,6.基金项目
国家重点研发计划项目(2023YFD1801200) （2023YFD1801200）
河南省科技厅动物源重要人兽共患病源头防控措施与关键技术创新项目(231111111500) （231111111500）
