中国蔬菜Issue(1)：71-84,14.DOI:10.19928/j.cnki.1000-6346.2025.3051
豇豆种质资源的KASP分子标记鉴定与遗传分析
Molecular Identification and Genetic Analysis of Cowpea Germplasm Resources Based on KASP Markers
摘要
Abstract
Cowpea[Vigna unguiculata(L.)Walp.]is a major leguminous vegetable in China.Studies on genotype identification and genetic diversity of its germplasm resources are of great significance for the conservation,exploration,and utilization of genetic resources.In this study,46 KASP molecular markers were used to detect and analyze 202 cowpea accessions.The results showed that the average gene diversity(GD)was 0.39(with a variation range of 0.03-0.52),the polymorphism information content(PIC)value was 0.32(with a variation range of 0.03-0.42),and the average major allele frequency(MAF)was 0.69(with a variation range of 0.50-0.99).This indicates that the genetic diversity of the tested cowpea materials is moderately low,which may restrict breeding potential.STRUCTURE analysis divided them into 3 subpopulations with relatively close genetic distances(0.01-0.02).Neighbor-joining(NJ)clustering classified all the cowpea accessions into 4 groups,among which group Ⅰ is mainly local varieties and group Ⅱis mainly commercial varieties.Based on these KASP markers,this study constructed molecular fingerprints of 202 cowpea germplasms,revealed the main SNP composition types at KASP variation sites,and screened 18 representative cowpea germplasms as mini-core germplasm resources,providing a scientific basis for the molecular conservation and utilization of cowpea resources.关键词
豇豆/KASP/SNP/基因分型/遗传多样性Key words
cowpea/KASP/SNP/genotyping/genetic diversity引用本文复制引用
梅翔,苏晓佳,朱姝萌,胡格格,吴新义,李国景,潘磊..豇豆种质资源的KASP分子标记鉴定与遗传分析[J].中国蔬菜,2026,(1):71-84,14.基金项目
国家自然科学基金项目(31501369),湖北省科技创新基地(平台)计划项目(2024CFA018),湖北省科技计划自然科学基金面上项目(2017CFB442),湖北省食用豆类植物自然科技资源中心主任基金项目(2017-ZR01),湖北省豆类(蔬菜)植物工程技术研究中心开放基金项目(2019-01) （31501369）
