中国麻业科学2025，Vol.47Issue(6)：310-321,12.
响应面法优化提取柑橘果皮类黄酮及其抗氧化活性研究
Optimization of the Extraction of Citrus Peel Flavonoids and Their Antioxidant Activity by Response Surface Methodology
摘要
Abstract
The citrus varieties of the highest hesperidin and naringin contents were screened out from 15 citrus species in the resource nursery,and the optimization of the extraction process and the study on antioxidant activity were carried out.The results showed that the varieties with the highest contents of hesperidin and naringin were Mihaya and grapefruit,respectively.The optimal conditions for the extraction of hesperidin and naringin were as follows:for hesperidin,solid-liquid ratio of 1∶36.19(g/mL),ultrasonic temperature of 45.20℃,and ultrasonic time of 47.47 min;for naringin,solid-liquid ratio of 1∶43.32(g/mL),ultrasonic temperature of 53.48℃,and ultrasonic time of 53.47 min.The predicted values of the yield of hesperidin and naringin were 12.75%and 8.31%,respectively.The antioxidant studies showed that the IC50 of scavenging DPPH radicals were 0.52 mg/mL and 5.57 mg/mL for hesperidin and naringin,respectively,while that of scavenging ABTS radicals were 0.41 mg/mL and 3.78 mg/mL.Moreover,0.8 mg/mL of hesperidin and 4.72 mg/mL of naringin in the ability to reduce Fe3+were roughly equivalent to 0.6 mg/mL of vitamin C.This study results in an optimal protocol for the extraction of hesperidin and naringin,and the antioxidant activity of hesperidin is higher than that of naringin.关键词
橙皮苷/柚皮苷/响应面法/工艺优化/抗氧化活性Key words
hesperidin/naringin/response surface methodology/process optimization/antioxidant activity分类
农业科技引用本文复制引用
高泽昕,柏云喜,谢淑婷,张广宝,常丽,栾明宝..响应面法优化提取柑橘果皮类黄酮及其抗氧化活性研究[J].中国麻业科学,2025,47(6):310-321,12.基金项目
中央公益性科研院所基本科研基金(1610242024010) （1610242024010）
中央级公益性科研院所基本科研业务费专项(Y2024ZK05) （Y2024ZK05）
中国农业科学院农业科技创新工程(2017IBFC) （2017IBFC）
中国农业科学院国家南繁研究所(三亚)(YBXM2420) （三亚）
幼龄柑橘园间作棉花增产增效栽培技术研究(2024-1) （2024-1）
